Transcriptome analysis of multiple tissues reveals the potential mechanism of death under acute heat stress in chicken

BMC Genomics

Research

Jiuhong Nan¹  Li Rong¹  Zijia Jia¹  Hongrui Yang¹  Sendong Yang¹  Shijun Li² 

[1]State Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China

[2]State Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China

[3]Hubei Hongshan Laboratory, Huazhong Agricultural University, 430070, Wuhan, China

[4]Key Laboratory of Smart Farming for Agricultural Animals, Ministry of Education, Huazhong Agricultural University, 430070, Wuhan, Hubei Province, China

关键词: Chicken;    Acute heat stress;    Death;    Lung;    Inflammation;   

DOI  :  10.1186/s12864-023-09564-2

 received in 2022-12-12, accepted in 2023-08-08,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundAcute heat stress could induce high mortality and cause huge economic losses in the poultry industry. Although many studies have revealed heat stress-induced injuries of multiple tissues, the main target tissue and molecular mechanism of death under acute heat stress was largely unknown. This study systematically compared the transcriptome data of five main visceral tissues in chickens to reveal the response of multiple tissues to acute heat stress and determine the main target tissue of acute heat stress, further revealing the injuries of main target tissue and their potential mechanism by combing pathological section and qRT-PCR technologies.ResultsThe transcriptome data of five visceral tissues revealed that acute heat stress broadly caused inflammatory response and damaged tissues metabolic homeostasis. Among the five tested visceral tissues, the number of differentially expressed genes in the lung was the highest, and their fold changes were the greatest, indicating that the lung was the main target tissue of acute heat stress. The results of pathological section revealed severe inflammation, emphysema and pulmonary hemorrhage in the lung under acute heat stress. Our study found that some pro-inflammatory genes, including CNTFR, FURIN, CCR6, LIFR and IL20RA, were significantly up-regulated both in the heat-stress and heat-death groups, and their fold changes in the heat-death group were significantly greater than that in the heat-stress group. We also found an anti-inflammatory gene, AvBD9, exhibiting an extremely high expression in the heat-stress group but a low expression in the heat-death group.ConclusionsOur study found that acute heat stress caused multiple tissue injuries broadly and the lung was the main target tissue of acute heat stress in chicken. Acute heat stress caused a severe inflammatory response, emphysema, and pulmonary haemorrhage, The severe inflammatory response in the heat-death group was related to the up-regulation of pro-inflammatory genes and down-regulation of anti-inflammatory genes.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

【 图 表 】

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