KRT18 regulates trophoblast cell migration and invasion which are essential for embryo implantation

Reproductive Biology and Endocrinology

Research

Mengying Liu¹  Sijia Chen¹  Yana Ma¹  Xiaoling Liang²  Xiaoxiao Qiu³  Peipei Zhang⁴  Xiaona Lin⁵  Wenzhi Xu⁵ 

[1]Assisted Reproduction Unit, Department of Obstetrics and Gynaecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China

[2]Assisted Reproduction Unit, Department of Obstetrics and Gynaecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China

[3]Assisted Reproduction Center, Northwest Women’s and Children’s Hospital, Xi’an, China

[4]Assisted Reproduction Unit, Department of Obstetrics and Gynaecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China

[5]Department of Obstetrics and Gynaecology, Taizhou Municipal Hospital, Taizhou, China

[6]Assisted Reproduction Unit, Department of Obstetrics and Gynaecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China

[7]Department of Obstetrics and Gynaecology, Tiantai People’s Hospital of Zhejiang Province, Taizhou, China

[8]Assisted Reproduction Unit, Department of Obstetrics and Gynaecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China

[9]Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province, Hangzhou, China

关键词: KRT18;    Embryo implantation;    Embryo adhesion;    E-cadherin;    Cell migration;    Cell invasion;   

DOI  :  10.1186/s12958-023-01129-y

 received in 2023-01-22, accepted in 2023-08-16,  发布年份 2023

来源: Springer

【 摘 要 】

Female infertility is a worldwide concern that impacts the quality of life and well-being of affected couples. Failure of embryo implantation is a major cause of early pregnancy loss and is precisely regulated by a programmed molecular mechanism. Recent studies have shown that proper trophoblast adhesion and invasion are essential for embryo implantation. However, the potential regulatory mechanism involved in trophoblast adhesion and invasion has yet to be fully elucidated. KRT18 has been reported to play a critical role in early embryonic development, but its physiological function in embryo implantation remains unclear. In the present study, we revealed that KRT18 was highly expressed in trophoblast cells and that knockdown of KRT18 in mouse embryos inhibited embryo adhesion and implantation. In vitro experiments further showed that silencing KRT18 disturbed trophoblast migration and invasion. More importantly, we provide evidence that KRT18 directly binds to and stabilizes cell surface E-cadherin in trophoblast cells through microscale thermophoresis (MST) analysis and molecular biology experiments. In brief, our data reveal that KRT18, which is highly expressed in trophoblast cells, plays an important role in the regulation of trophoblast invasion and adhesion during embryo implantation by directly binding to E-cadherin.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

【 图 表 】

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