A novel biocontrol agent Bacillus velezensis K01 for management of gray mold caused by Botrytis cinerea

AMB Express

Original Article

Qiaolin Xu¹  Yinting Xue¹  Yanbin Guo¹  Yunge Zhang²  Mingzhen Xing²  Xiuyan Wang²  Kun Huang² 

[1] College of Resources and Environmental Sciences, China Agricultural University, 100193, Beijing, China;Hebei Innovation Center of Biofertilizer Technology, 054700, Xingtai, Hebei, China;

关键词: Bacillus velezensis;    Biocontrol efficiency;    Botrytis cinerea;    Antagonistic activities;    Secondary metabolite;   

DOI  :  10.1186/s13568-023-01596-x

 received in 2023-08-04, accepted in 2023-08-12,  发布年份 2023

来源: Springer

【 摘 要 】

Gray mold is a destructive plant disease caused by a fungal pathogen Botrytis cinerea. The use of plant growth promoting rhizobacteria (PGPR) has proven to be a promising method to control this disease. Bacillus velezensis K01 was isolated from the rhizosphere of planting tomatoes. Strain K01 has a range of roles, including the ability to solubilize phytate phosphorus, stimulate resistant response, and produce indoleacetic acid (IAA), protease, cellulase, and antimicrobial substances. Strain K01 was found to inhibit 12 phytopathogenic fungi and 5 phytopathogenic bacteria. Specially, strain K01 demonstrated a biocontrol efficiency of over 78% against gray mold caused by B. cinerea on the leaves and fruits of tomato and pepper. Additionally, K01 was found to promote the growth of maize seedlings. Further genomic analysis revealed that K01 belongs to B. velezensis, which is consistent with phylogenetic analysis, average nucleotide polymorphism (ANI), and digital DNA–DNA hybridization (dDDH). The genome of strain K01 had a size of 3,927,799 bp and deduced 3866 predicted genes, with an average guanine-cytosine (GC) content of 46.5%. Based on the analyses of genomic secondary metabolites, over 18.4% of the genome was annotated to 12 gene clusters related to antimicrobial metabolite synthesis. Additionally, genome annotation and comparative genomics identified several genes associated with plant growth promotion and environmental adaption. These findings suggest that B. velezensis K01 has the potential to serve as a new biocontrol agent for management of gray mold on tomato and pepper.

【 授权许可】

CC BY   
© Springer-Verlag GmbH Germany, part of Springer Nature 2023

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