Preliminary study on toxicological mechanism of golden cuttlefish (Sepia esculenta) larvae exposed to cd

BMC Genomics

Research

Xiumei Liu¹  Xibo Zhu²  Jianmin Yang³  Zan Li³  Xiaokai Bao³ 

[1] College of Life Sciences, Yantai University, 264005, Yantai, China;Fishery Technology Service Center of Lanshan District, 276800, Rizhao, China;School of Agriculture, Ludong University, 264025, Yantai, China;

关键词: Cd;    Neurotoxicity;    Oxidative stress;    Protein-protein interaction network;    Sepia esculenta;    Transcriptome;   

DOI  :  10.1186/s12864-023-09630-9

 received in 2023-06-18, accepted in 2023-08-27,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundCadmium (Cd) flows into the ocean with industrial and agricultural pollution and significantly affects the growth and development of economic cephalopods such as Sepia esculenta, Amphioctopus fangsiao, and Loligo japonica. As of now, the reasons why Cd affects the growth and development of S. esculenta are not yet clear.ResultsIn this study, transcriptome and four oxidation and toxicity indicators are used to analyze the toxicological mechanism of Cd-exposed S. esculenta larvae. Indicator results indicate that Cd induces oxidative stress and metal toxicity. Functional enrichment analysis results suggest that larval ion transport, cell adhesion, and some digestion and absorption processes are inhibited, and the cell function is damaged. Comprehensive analysis of protein-protein interaction network and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis was used to explore S. esculenta larval toxicological mechanisms, and we find that among the 20 identified key genes, 14 genes are associated with neurotoxicity. Most of them are down-regulated and enriched to the neuroactive ligand-receptor interaction signaling pathway, suggesting that larval nervous system might be destroyed, and the growth, development, and movement process are significantly affected after Cd exposure.ConclusionsS. esculenta larvae suffered severe oxidative damage after Cd exposure, which may inhibit digestion and absorption functions, and disrupt the stability of the nervous system. Our results lay a function for understanding larval toxicological mechanisms exposed to heavy metals, promoting the development of invertebrate environmental toxicology, and providing theoretical support for S. esculenta artificial culture.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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