Mechanism of inulin in colic and gut microbiota of captive Asian elephant

Microbiome

Brief Report

Yuqi Wang¹  Yipeng Cong¹  Yi Luo¹  Zheng Wang¹  He Liu¹  Tianchun Pu¹  Lu Wang¹  Bo Yu¹  Tingbei Bo²  Qingduo Li³  Qiyong Liu⁴  Min Liu⁵  Dehua Wang⁶ 

[1] Beijing key laboratory of captive wildlife technology, Beijing Zoo, 100044, Beijing, China;School of Grassland Science, Beijing Forestry University, 100091, Beijing, China;State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China;State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China;State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China;Department of Vector Control, School of Public Health, Shandong University, 250012, Jinan, China;State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China;CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, 100049, Beijing, China;State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China;School of Life Science, Shandong University, 266237, Qingdao, China;

关键词: Inulin;    Colic;    Gut microbiota;    Elephas maximus;   

DOI  :  10.1186/s40168-023-01581-3

 received in 2022-04-28, accepted in 2023-05-23,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundGut microbiota have a complex role on the survivability, digestive physiology, production, and growth performance in animals. Recent studies have emphasized the effects of prebiotics therapy on the gut disease, but the relationship between elephant gut-related diseases and prebiotics remains elusive. Here, a case study was undertaken to evaluate the mechanism of inulin treatment in colic in Asian elephant (Elephas maximus Linnaeus).MethodsFecal samples were collected from a sick elephant and four healthy elephants. Analysis of microbial profile was carried out by 16S rRNA sequencing, and the short chain fatty acids were tested by gas chromatography. The physiological function of “inulin-microbiota” of elephant was verified in mice by fecal microbial transplantation (FMT). The expression of related proteins was determined by Western blotting and qPCR.Results(1) Eating inulin can cure gut colic of the sick elephant and changed gut microbiota. (2) It was found that “inulin microbiota” from the post-treatment elephants can promote the proliferation of intestinal cells, increase the utilization of short chain fatty acids (SCFAs), maintain intestinal barrier, and reduce the inflammation in mice. (3) The mechanism was inulin—gut microbiota—SCFAs—immune barrier.ConclusionsInulin contributed to rehabilitate the gut microbiota and gut immune barrier of the elephant with colic. This provides reasonable verification for using prebiotics to treat the colic in captive elephants.Prebiotics will foresure play an increasingly important role in disease prevention and treatment of captive animals in the future.Graphical Abstract6TxumGndbMVxR2Rm3P8oQ1Video Abstract

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

41116_2023_38_Article_IEq237.gif

41116_2023_38_Article_IEq235.gif

41116_2023_38_Article_IEq228.gif

41116_2023_38_Article_IEq222.gif

41116_2023_38_Article_IEq217.gif

41116_2023_38_Article_IEq213.gif

Fig. 2

41116_2023_38_Article_IEq181.gif

41116_2023_38_Article_IEq157.gif