Rhizobial nitrogen fixation efficiency shapes endosphere bacterial communities and Medicago truncatula host growth

Microbiome

Research

Bethany L. Richmond¹  Beatriz Lagunas¹  Jamie Burgess¹  Gina Stovold¹  Luke Richards¹  Anastasia Pakidi¹  Chrysi Sergaki¹  Rana M. F. Hussain¹  Proyash Roy²  Patrick Schäfer³  Miriam L. Gifford⁴  Laura Baxter⁵  Saúl Vázquez⁶  Alonso Javier Pardal⁷  Sascha Ott⁷ 

[1]School of Life Sciences, University of Warwick, CV4 7AL, Coventry, UK

[2]School of Life Sciences, University of Warwick, CV4 7AL, Coventry, UK

[3]Department of Genetic Engineering & Biotechnology, University of Dhaka, Dhaka, Bangladesh

[4]School of Life Sciences, University of Warwick, CV4 7AL, Coventry, UK

[5]Present Address: Institute of Phytopathology, Research Centre for BioSystems, Land Use and Nutrition, Justus Liebig University, 35392, Giessen, Germany

[6]School of Life Sciences, University of Warwick, CV4 7AL, Coventry, UK

[7]Warwick Integrative Synthetic Biology Centre, University of Warwick, CV47AL, Coventry, UK

[8]School of Life Sciences, University of Warwick, CV4 7AL, Coventry, UK

[9]Warwick Medical School, University of Warwick, CV4 7AL, Coventry, UK

[10]University of Nottingham, Sutton Bonington Campus, Sutton Bonington, LE12 5RD, Nottingham, UK

[11]Warwick Medical School, University of Warwick, CV4 7AL, Coventry, UK

关键词: Medicago truncatula;    Nodulation;    Plant–rhizobial interaction;    Nitrogen fixation efficiency;    Soil;    Endosphere;    Microbial communities;    Nodule sanctioning;   

DOI  :  10.1186/s40168-023-01592-0

 received in 2022-12-31, accepted in 2023-06-05,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundDespite the knowledge that the soil–plant–microbiome nexus is shaped by interactions amongst its members, very little is known about how individual symbioses regulate this shaping. Even less is known about how the agriculturally important symbiosis of nitrogen-fixing rhizobia with legumes is impacted according to soil type, yet this knowledge is crucial if we are to harness or improve it. We asked how the plant, soil and microbiome are modulated by symbiosis between the model legume Medicago truncatula and different strains of Sinorhizobium meliloti or Sinorhizobium medicae whose nitrogen-fixing efficiency varies, in three distinct soil types that differ in nutrient fertility, to examine the role of the soil environment upon the plant–microbe interaction during nodulation.ResultsThe outcome of symbiosis results in installment of a potentially beneficial microbiome that leads to increased nutrient uptake that is not simply proportional to soil nutrient abundance. A number of soil edaphic factors including Zn and Mo, and not just the classical N/P/K nutrients, group with microbial community changes, and alterations in the microbiome can be seen across different soil fertility types. Root endosphere emerged as the plant microhabitat more affected by this rhizobial efficiency-driven community reshaping, manifested by the accumulation of members of the phylum Actinobacteria. The plant in turn plays an active role in regulating its root community, including sanctioning low nitrogen efficiency rhizobial strains, leading to nodule senescence in particular plant–soil–rhizobia strain combinations.ConclusionsThe microbiome–soil–rhizobial dynamic strongly influences plant nutrient uptake and growth, with the endosphere and rhizosphere shaped differentially according to plant–rhizobial interactions with strains that vary in nitrogen-fixing efficiency levels. These results open up the possibility to select inoculation partners best suited for plant, soil type and microbial community.Dv-bX6Q_Z8DTbrQMTFWdQvVideo Abstract

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

41116_2023_38_Article_IEq151.gif

41116_2023_38_Article_IEq146.gif

Fig. 3

41116_2023_38_Article_IEq130.gif

41116_2023_38_Article_IEq128.gif

41116_2023_38_Article_IEq126.gif

41116_2023_38_Article_IEq124.gif

41116_2023_38_Article_IEq122.gif

Fig. 1

41116_2023_38_Article_IEq117.gif

41116_2023_38_Article_IEq114.gif

41116_2023_38_Article_IEq110.gif

41116_2023_38_Article_IEq99.gif

41116_2023_38_Article_IEq94.gif

41116_2023_38_Article_IEq87.gif

41116_2023_38_Article_IEq78.gif

41116_2023_38_Article_IEq72.gif

41116_2023_38_Article_IEq57.gif

41116_2023_38_Article_IEq51.gif