Intrahippocampal Inoculation of A beta(1-42) Peptide in Rat as a Model of Alzheimer's Disease Identified MicroRNA-146a-5p as Blood Marker with Anti-Inflammatory Function in Astrocyte Cells

卷:12

Article

关键词: NF-KAPPA-B;    CEREBROSPINAL-FLUID;    CIRCULATING MICRORNAS;    PERIPHERAL BIOMARKERS;    AGE;    EXPRESSION;    INHIBITOR;    PROTEINS;    GENES;    IRAK1;   

DOI  :  10.3390/cells12050694

来源: SCIE

【 摘 要 】

Circulating microRNAs (miRNAs) have aroused a lot of interest as reliable blood diagnostic biomarkers of Alzheimer's disease (AD). Here, we investigated the panel of expressed blood miRNAs in response to aggregated A beta(1-42) peptides infused in the hippocampus of adult rats to mimic events of the early onset of non-familial AD disorder. A beta(1-42) peptides in the hippocampus led to cognitive impairments associated with an astrogliosis and downregulation of circulating miRNA-146a-5p, -29a-3p, -29c-3p, -125b-5p, and-191-5p. We established the kinetics of expression of selected miRNAs and found differences with those detected in the APP(swe)/PS1(dE9) transgenic mouse model. Of note, miRNA-146a-5p was exclusively dysregulated in the A beta-induced AD model. The treatment of primary astrocytes with A beta(1-42) peptides led to miRNA-146a-5p upregulation though the activation of the NF-kappa B signaling pathway, which in turn downregulated IRAK-1 but not TRAF-6 expression. As a consequence, no induction of IL-1 beta, IL-6, or TNF-alpha was detected. Astrocytes treated with a miRNA-146-5p inhibitor rescued IRAK-1 and changed TRAF-6 steady-state levels that correlated with the induction of IL-6, IL-1 beta, and CXCL1 production, indicating that miRNA-146a-5p operates anti-inflammatory functions through a NF-kappa B pathway negative feedback loop. Overall, we report a panel of circulating miRNAs that correlated with A beta(1-42) peptides' presence in the hippocampus and provide mechanistic insights into miRNA-146a-5p biological function in the development of the early stage of sporadic AD.