Structural Transition of the Nucleosome during Transcription Elongation

卷:12

Review

关键词: RNA-POLYMERASE-II;    FACTORS REPRESS TRANSCRIPTION;    CHROMATIN-STRUCTURE;    HISTONE MODIFICATIONS;    CRYSTAL-STRUCTURE;    SIN MUTATIONS;    FACT COMPLEX;    DNA;    DOMAIN;    SPT16;   

DOI  :  10.3390/cells12101388

来源: SCIE

【 摘 要 】

In eukaryotes, genomic DNA is tightly wrapped in chromatin. The nucleosome is a basic unit of chromatin, but acts as a barrier to transcription. To overcome this impediment, the RNA polymerase II elongation complex disassembles the nucleosome during transcription elongation. After the RNA polymerase II passage, the nucleosome is rebuilt by transcription-coupled nucleosome reassembly. Nucleosome disassembly-reassembly processes play a central role in preserving epigenetic information, thus ensuring transcriptional fidelity. The histone chaperone FACT performs key functions in nucleosome disassembly, maintenance, and reassembly during transcription in chromatin. Recent structural studies of transcribing RNA polymerase II complexed with nucleosomes have provided structural insights into transcription elongation on chromatin. Here, we review the structural transitions of the nucleosome during transcription.