Pathogenic Th2 Cytokine Profile Skewing by IFN-gamma-Responding Vitiligo Fibroblasts via CCL2/CCL8

卷:12

Article

关键词: MONOCYTE CHEMOATTRACTANT PROTEIN-1;    T-CELLS;    SKIN PIGMENTATION;    EXPRESSION;    CHEMOKINES;    CCL2;    POLARIZATION;    MEMORY;   

DOI  :  10.3390/cells12020217

来源: SCIE

【 摘 要 】

Purpose: Vitiligo is a T cell-mediated skin depigmentation disease. Though treatments arresting disease progression and inducing repigmentation are available, the efficacy of these options is often limited and poorly sustained. How stromal signals contribute to the interferon-gamma-dominant skin niches is unclear. This study aims to determine how fibroblasts participate in the IFN-gamma-dominant vitiligo niche. Patients and methods: Mouse vitiligo models were established. Fibroblasts from control and vitiligo mice were extracted for RNA sequencing. In vitro IFN-gamma stimulation was performed to verify the JAK-STAT pathway by qPCR and Western blot. T cell polarization with chemokines was measured by flow cytometry. Protein levels in tissues were also examined by IHC. Results: The vitiligo mouse model recapitulates the human CD8-IFN-gamma pathway. RNA sequencing revealed elevated chemokine CCL2 and CCL8 in vitiligo fibroblast, which may be regulated by the JAK-STAT signaling. Such phenomenon is verified by JAK inhibitor peficitinib in vitro. Moreover, CCL2 addition into the naive T polarization system promoted type 2 cytokines secretion, which represents a hallmark of vitiligo lesions. Conclusion: Dermal fibroblasts, a principal constituent of skin structure, respond to IFN-gamma by skewing T cells towards a type 2 cytokine profile via CCL2 and CCL8, which can be abrogated by JAK inhibitor peficitinib.