Analysis of the Anticipatory Behavior Formation Mechanism Induced by Methamphetamine Using a Single Hair

卷:12

Article

关键词: PERIOD1 GENE-EXPRESSION;    CIRCADIAN CLOCK GENES;    OLFACTORY-BULB;    FIREFLY LUCIFERASE;    LOCOMOTOR-ACTIVITY;    ACTIVITY RHYTHM;    OSCILLATOR;    BRAIN;    LIVER;    PER2;   

DOI  :  10.3390/cells12040654

来源: SCIE

【 摘 要 】

While the suprachiasmatic nucleus (SCN) coordinates many daily rhythms, some circadian patterns of expression are controlled by SCN-independent systems. These include responses to daily methamphetamine (MAP) injections. Scheduled daily injections of MAP resulted in anticipatory activity, with an increase in locomotor activity immediately prior to the time of injection. The MAP-induced anticipatory behavior is associated with the induction and a phase advance in the expression rhythm of the clock gene Period1 (Per1). However, this unique formation mechanism of MAP-induced anticipatory behavior is not well understood. We recently developed a micro-photomultiplier tube (micro-PMT) system to detect a small amount of Per1 expression. In the present study, we used this system to measure the formation kinetics of MAP-induced anticipatory activity in a single whisker hair to reveal the underlying mechanism. Our results suggest that whisker hairs respond to daily MAP administration, and that Per1 expression is affected. We also found that elevated Per1 expression in a single whisker hair is associated with the occurrence of anticipatory behavior rhythm. The present results suggest that elevated Per1 expression in hairs might be a marker of anticipatory behavior formation.