Mitochondrial Haemoglobin Is Upregulated with Hypoxia in Skeletal Muscle and Has a Conserved Interaction with ATP Synthase and Inhibitory Factor 1

卷:12

Article

关键词: ANTIFREEZE GLYCOPROTEINS;    DROSOPHILA-MELANOGASTER;    ORGANIC-PHOSPHATES;    NITRIC-OXIDE;    EXPRESSION;    GLOBIN;    BLOOD;    GENE;    EVOLUTION;    MYOGLOBIN;   

DOI  :  10.3390/cells12060912

来源: SCIE

【 摘 要 】

The globin protein superfamily has diverse functions. Haemoglobin has been found in non-erythroid locations, including within the mitochondria. Using co-immunoprecipitation and in silico methods, we investigated the interaction of mitochondrial haemoglobin with ATP synthase and its associated proteins, including inhibitory factor 1 (IF1). We measured the expression of mitochondrial haemoglobin in response to hypoxia. In vitro and in silico evidence of interactions between mitochondrial haemoglobin and ATP synthase were found, and we report upregulated mitochondrial haemoglobin expression in response to hypoxia within skeletal muscle tissue. Our observations indicate that mitochondrial pH and ATP synthase activity are implicated in the mitochondrial haemoglobin response to hypoxia.