CLOCK inhibits the proliferation of porcine ovarian granulosa cells by targeting ASB9

Journal of Animal Science and Biotechnology

Research

Gongshe Yang¹  Weijun Pang¹  Liang Huang¹  Lutong Zhang¹  Guiyan Chu¹  Huan Yuan¹  Xiaoge Zhou¹  Xiangrong Song¹  Lei Gao¹  Shengjie Shi¹ 

[1] Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, 712100, Yangling, China;Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, China;

关键词: ASB9;    CLOCK;    Granulosa cells;    Pig;    Proliferation;   

DOI  :  10.1186/s40104-023-00884-7

 received in 2023-01-02, accepted in 2023-04-16,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundClock circadian regulator (CLOCK) is a core factor of the mammalian biological clock system in regulating female fertility and ovarian physiology. However, CLOCK's specific function and molecular mechanism in porcine granulosa cells (GCs) remain unclear. In this study, we focused on CLOCK’s effects on GC proliferation.ResultsCLOCK significantly inhibited cell proliferation in porcine GCs. CLOCK decreased the expression of cell cycle-related genes, including CCNB1, CCNE1, and CDK4 at the mRNA and protein levels. CDKN1A levels were upregulated by CLOCK. ASB9 is a newly-identified target of CLOCK that inhibits GC proliferation; CLOCK binds to the E-box element in the ASB9 promoter.ConclusionsThese findings suggest that CLOCK inhibits the proliferation of porcine ovarian GCs by increasing ASB9 level.

【 授权许可】

CC BY   
© The Author(s) 2023

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