Immunohistochemical detection of MUC5AC and MUC5B mucins in ferrets

BMC Research Notes

Research Note

John F. Engelhardt¹  Yan Zhang¹  J. Adam Goeken²  Mariah R. Leidinger²  Idil Evans²  Allison Akers²  Thomas R. Businga²  David K. Meyerholz²  Sebastian Vizuett² 

[1] Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, 52242, Iowa City, IA, USA;Department of Pathology, Carver College of Medicine, University of Iowa, 52242, Iowa City, IA, USA;

关键词: Cystic fibrosis;    Ferret;    Immunohistochemistry;    Lung;    Mucin;    Mucus;    MUC5AC;    MUC5B;    Tissue;    Pathology;   

DOI  :  10.1186/s13104-023-06388-x

 received in 2023-01-10, accepted in 2023-06-15,  发布年份 2023

来源: Springer

【 摘 要 】

ObjectiveCystic fibrosis (CF) is a genetic condition that causes abnormal mucus secretions in affected organs. MUC5AC and MUC5B are gel-forming mucins and frequent targets for investigations in CF tissues. Our objective was to qualify MUC5AC and MUC5B immunohistochemical techniques to provide a useful tool to identify, localize and interpret mucin expression in ferret tissues.ResultsMUC5AC and MUC5B mucins were detected most commonly in large airways and least in small airways, consistent with reported goblet cell density in airway surface epithelia. We evaluated whether staining method affected the detection of goblet cell mucins in serial sections of bronchial surface epithelia. Significant differences between stains were not observed suggesting common co-expression MUC5AC and MUC5B proteins in goblet cells of airway surface epithelia. Gallbladder and stomach tissues are reported to have differential mucin enrichment, so we tested these tissues in wildtype ferrets. Stomach tissues were enriched in MUC5AC and gallbladder tissues enriched in MUC5B, mucin enrichment similar to human tissues. Mucin immunostaining techniques were further qualified for specificity using lung tissue from recently generated MUC5AC−/− and MUC5B−/− ferrets. Qualified techniques for MUC5AC and MUC5B immunohistochemistry will be useful tools for mucin tissue studies in CF and other ferret models.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

40517_2023_252_Article_IEq73.gif

40517_2023_252_Article_IEq29.gif