Comparative transcriptomic analysis of male and females in the dioecious weeds Amaranthus palmeri and Amaranthus tuberculatus

BMC Plant Biology

Research

Lucas K. Bobadilla¹  Patrick J. Tranel¹  Yousoon Baek¹ 

[1] Department of Crop Sciences, University of Illinois, Urbana, IL, USA;

关键词: Dioecy;    Sex-determination;    Reproduction;    Weeds;    RNA-seq;    Sex-determinant region;    Waterhemp;    Palmer amaranth;   

DOI  :  10.1186/s12870-023-04286-9

 received in 2023-02-27, accepted in 2023-05-16,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundWaterhemp (Amaranthus tuberculatus (Moq.) Sauer) and Palmer amaranth (Amaranthus palmeri S. Wats.) are two dioecious and important weed species in the world that can rapidly evolve herbicide-resistance traits. Understanding these two species' dioecious and sex-determination mechanisms could open opportunities for new tools to control them. This study aims to identify the differential expression patterns between males and females in A. tuberculatus and A. palmeri. Multiple analyses, including differential expression, co-expression, and promoter analyses, used RNA-seq data from multiple tissue types to identify putative essential genes for sex determination in both dioecious species.ResultsGenes were identified as potential key players for sex determination in A. palmeri. Genes PPR247, WEX, and ACD6 were differentially expressed between the sexes and located at scaffold 20 within or near the male-specific Y (MSY) region. Multiple genes involved with flower development were co-expressed with these three genes. For A. tuberculatus, no differentially expressed gene was identified within the MSY region; however, multiple autosomal class B and C genes were identified as differentially expressed and possible candidate genes.ConclusionsThis is the first study comparing the global expression profile between males and females in dioecious weedy Amaranthus species. Results narrow down putative essential genes for sex-determination in A. palmeri and A. tuberculatus and also strengthen the hypothesis of two different evolutionary events for dioecy within the genus.

【 授权许可】

CC BY   
© The Author(s) 2023

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