Biocatalysis of triglycerides transesterification using fungal biomass: a biorefinery approach

Fungal Biology and Biotechnology

Research

Ahmed M. A. Mohamed¹  Sayeda S. Mohamed²  Heba A. El-Refai²  Hala A. Amin²  Nadeem I. Elhussiny³  Yousseria M. Shetaia⁴  Gerd Klöck⁵ 

[1] Department of Botany and Microbiology, Faculty of Science, Helwan University, 11795, Cairo, Egypt;Department of Chemistry of Natural and Microbial Products, National Research Centre, Dokki, 12622, Cairo, Egypt;Department of Life Science and Chemistry, Constructor University, Bremen Campus Ring 1, 28759, Bremen, Germany;Department of Chemistry of Natural and Microbial Products, National Research Centre, Dokki, 12622, Cairo, Egypt;Institute of Environmental Biology and Biotechnology, University of Applied Sciences, 28199, Bremen, Germany;Department of Microbiology, Faculty of Science, Ain Shams University, Abbassia, 11566, Cairo, Egypt;Institute of Environmental Biology and Biotechnology, University of Applied Sciences, 28199, Bremen, Germany;

关键词: Biorefinery;    Transesterification;    Aspergillus flavus;    Rhizopus stolonifer;    Wastewater;    Fungal biomass;    Biocatalysis;   

DOI  :  10.1186/s40694-023-00160-3

 received in 2023-03-12, accepted in 2023-05-11,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe use of microbial biomasses, such as fungal biomass, to catalyze the transesterification of triglycerides (TG) for biodiesel production provides a sustainable, economical alternative while still having the main advantages of expensive immobilized enzymes.ResultsBiomasses of Aspergillus flavus and Rhizopus stolonifera were used to catalyze the transesterification of TG in waste frying oil (WFO). Isopropanol as an acyl-acceptor reduced the catalytic capability of the biomasses, while methanol was the most potent acyl-acceptor with a final fatty acid methyl ester (FAME) concentration of 85.5 and 89.7%, w/w, for R. stolonifer and A. flavus, respectively. Different mixtures of the fungal biomasses were tested, and higher proportions of A. flavus biomass improved the mixture's catalytic capability. C. sorokiniana cultivated in synthetic wastewater was used as feedstock to cultivate A. flavus. The biomass produced had the same catalytic capability as the biomass produced in the control culture medium. Response surface methodology (RSM) was adopted using central composite design (CCD) to optimize the A. flavus biomass catalytic transesterification reaction, where temperature, methanol concentration, and biomass concentration were selected for optimization. The significance of the model was verified, and the suggested optimum reaction conditions were 25.5 °C, 250 RPM agitation with 14%, w/w, biomass, 3 mol/L methanol, and a reaction duration of 24 h. The suggested optimum conditions were tested to validate the model and a final FAME concentration of 95.53%. w/w was detected.ConclusionBiomasses cocktails might be a legitimate possibility to provide a cheaper technical solution for industrial applications than immobilized enzymes. The use of fungal biomass cultivated on the microalgae recovered from wastewater treatment for the catalysis of transesterification reaction provides an additional piece of the puzzle of biorefinery. Optimizing the transesterification reaction led to a valid prediction model with a final FAME concentration of 95.53%, w/w.

【 授权许可】

CC BY   
© The Author(s) 2023

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