Theranostic RGD@Fe3O4-Au/Gd NPs for the targeted radiotherapy and MR imaging of breast cancer

Cancer Nanotechnology

Research

Hossein Ghadiri Harvani¹  Abolfazl Sarikhani²  Zahra Alamzadeh²  Rasoul Irajirad²  Samideh Khoei³  Seied Rabi Mahdavi⁴  Sakine Shirvalilou⁵  Azadeh Amraee⁵  Habib Ghaznavi⁶ 

[1]Department of Medical Physics and Biomedical Engineering, Tehran University of Medical Sciences, Tehran, Iran

[2]Finetech in Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran

[3]Finetech in Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran

[4]Medical Physics Department, School of Medicine, Iran University of Medical Sciences, P.O.Box: 1449614525, Tehran, Iran

[5]Medical Physics Department, School of Medicine, Iran University of Medical Sciences, P.O.Box: 1449614525, Tehran, Iran

[6]Medical Physics Department, School of Medicine, Iran University of Medical Sciences, P.O.Box: 1449614525, Tehran, Iran

[7]Finetech in Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran

[8]Pharmacology Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

[9]Cellular and Molecular Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran

关键词: Ultra-small iron oxide magnetic NPs;    Positive contrast;    T1-weighted MRI;    Radiation therapy;    Theranostics;    Breast cancer;   

DOI  :  10.1186/s12645-023-00214-6

 received in 2022-11-16, accepted in 2023-05-31,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundAs a radiosensitizing agent in magnetic resonance imaging (MRI), gadolinium is disadvantageous in that it confers a rather high toxicity and low longitudinal comfort time (r1). We hypothesized that gadolinium when combined with gold-coated iron oxide nanoparticles (NPs), might deliver better radiosensitization in MRI-based cancer theranostics. After being synthesized ligand/receptor RGD@Fe3O4-Au/Gd nanoparticles, they were characterized via some methods, such as visible–ultraviolet spectroscopy (UV–VIS), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), and transmission electron microscope (TEM). Using relaxometry, the parameters of contrast change in T1-weighted MRI and the rate of radiation sensitivity on cancerous (MCF-7, SK-BR-3 and MDA-MB-231 (and reference (MCF-10a) breast cell lines were investigated.ResultsThe presence of ultra-small iron oxide, gold, gadolinium, and RGD peptide as components of the RGD@Fe3O4-Au/Gd nanocomplex was confirmed by UV–visible, FTIR, EDX and XRD tests. With a size ranging from 4.124 nm (DLS) to 15 nm (TEM), these NPs exhibited a surface charge of –45.7 mV and a magnetic saturation of 3 emu/g. The concentrations of iron, gadolinium and gold samples in the nanocomplex were 1000, 1171 and 400 parts per million (ppm), respectively. In the relaxometry test, the rates of r2/r1 and r1 NPs were 1.56 and 23.5 mM−1 s−1. The dose increase factor for targeted (RGD@Fe3O4-Au/Gd) and non-targeted (Fe3O4-Au/Gd) NPs at 6 MV and 2 Gy was 89.1 and 59.1, respectively.ConclusionOwing to an enhanced signal-to-noise ratio (SNR), as confirmed by the MRI of RGD receptor-expressing MDA-MB-231 cells, RGD@Fe3O4-Au/Gd NPs were found to confer higher radiosensitization and an overall better performance as a novel radiosensitizer for MRI-based breast cancer theranostics than Fe3O4-Au/Gd nanocomplex.Graphical Abstract

【 授权许可】

CC BY   
© The Author(s) 2023

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