Three R2R3-MYB transcription factors from banana (Musa acuminata) activate structural anthocyanin biosynthesis genes as part of an MBW complex

BMC Research Notes

Research Note

Bernd Weisshaar¹  Ralf Stracke¹  Mareike Busche¹  Boas Pucker² 

[1] Genetics and Genomics of Plants, Faculty of Biology, Bielefeld University, 33615, Bielefeld, Germany;Institute of Plant Biology & Braunschweig Integrated Centre of Systems Biology (BRICS), TU Braunschweig, 38106, Braunschweig, Germany;

关键词: Banana;    Flavonoid biosynthesis;    Musa acuminata;    R2R3-MYB;    Specialised metabolites;    Anthocyanin;   

DOI  :  10.1186/s13104-023-06375-2

 received in 2022-08-22, accepted in 2023-06-02,  发布年份 2023

来源: Springer

【 摘 要 】

ObjectiveBananas are one of the most popular fruits in the world, providing food security and employment opportunities in several developing countries. Increasing the anthocyanin content of banana fruit could improve the health-promoting properties. Anthocyanin biosynthesis is largely regulated at the transcriptional level. However, relatively little is known about the transcriptional activation of anthocyanin biosynthesis in banana.ResultsWe analysed the regulatory activity of three Musa acuminata MYBs that were predicted by bioinformatic analysis to transcriptionally regulate anthocyanin biosynthesis in banana. MaMYBA1, MaMYBA2 and MaMYBPA2 did not complement the anthocyanin-deficient phenotype of the Arabidopsis thaliana pap1/pap2 mutant. However, co-transfection experiments in A. thaliana protoplasts showed that MaMYBA1, MaMYBA2 and MaMYBPA2 function as components of a transcription factor complex with a bHLH and WD40 protein, the so called MBW complex, resulting in the activation of the A. thaliana ANTHOCYANIDIN SYNTHASE and DIHYDROFLAVONOL 4-REDUCTASE promoters. The activation potential of MaMYBA1, MaMYBA2 and MaMYBPA2 was increased when combined with the monocot Zea mays bHLH ZmR instead of the dicot AtEGL3. This work paves the path towards decoding the MBW complex-mediated transcriptional activation of anthocyanin biosynthesis in banana. It will also facilitate research towards increased anthocyanin content in banana and other monocot crops.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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Fig. 1