Angiotensin-converting enzyme 2 (ACE2) polymorphisms and susceptibility of severe SARS-CoV-2 in a subset of Pakistani population

Virology Journal

Research

Omer Ahmed Shaikh¹  Rizma Khan²  Ambrina Khatoon³  Santosh Kumar Sidhwani⁴  Fouzia Shaikh⁴  Talat Mirza⁵  Abdulqadir J. Nashwan⁶ 

[1] Department of Medicine, Ziauddin University, Karachi, Pakistan;Department of Molecular Genetics, Ziauddin University, Karachi, Pakistan;Department of Molecular Medicine, Ziauddin University, Karachi, Pakistan;Department of Pathology, Ziauddin University, Karachi, Pakistan;Department of Research, Ziauddin University, Karachi, Pakistan;Department of Molecular Medicine, Ziauddin University, Karachi, Pakistan;Nursing for Education Hamad Medical Corporation, P.O. Box 3050, Doha, Qatar;

关键词: SARS-CoV-2;    ACE2;    Polymorphisms;    rs2285666;    Variants;    SNPs;   

DOI  :  10.1186/s12985-023-02091-2

 received in 2023-02-24, accepted in 2023-06-02,  发布年份 2023

来源: Springer

【 摘 要 】

Science is digging for the varied presentation of COVID-19 patients exposed to the same risk factors, and medical conditions may be influenced by the presence of polymorphic genetic variants. This study investigated the link between ACE2 gene polymorphisms and the severity of SARS-CoV-2. This cross-sectional study recruited COVID-19 PCR-positive patients by consecutive sampling from Ziauddin Hospital from April to September 2020. DNA was extracted from whole blood, followed by gene amplification and Sanger’s sequencing. Most of the patients, 77: 53.8%, were serious. Males were higher (80; 55.9%) with age more than 50 years (106: 74.1%). We found 22 ACE2 SNPs. rs2285666 SNP was most prevalent with 49.2% CC, 45.2% TT, 4.8% CT heterozygosity, and 0.8% AA genotypes. Variants with multiple genotypes were also insignificantly associated with the severity of COVID-19 in the analysis of the dominant model. Only rs2285666 had a significant statistical link with gender (p-value 0.034, OR; 1.438, CI; 1.028–2.011) while rs768883316 with age groups (p-value 0.026, OR; 1.953, CI; 1.085–3.514). Haplotypes ATC of three polymorphisms (rs560997634, rs201159862, and rs751170930) commonly found in 120 (69.77%) and TTTGTAGTTAGTA haplotype consisting of 13 polymorphisms (rs756737634, rs146991645, rs1601703288, rs1927830489, rs1927831624, rs764947941, rs752242172, rs73195521, rs781378335, rs756597390, rs780478736, rs148006212, rs768583671) in 112 (90.32%) had statistically significant association with the severity having p = value 0.029 and 0.001 respectively. Males of old age and diabetics are found to have more severe COVID-19 infection in the current study. We also found that common ACE2 polymorphism rs2285666 influences the susceptibility of acquiring the severe SARS-CoV-2 infection.

【 授权许可】

CC BY   
© The Author(s) 2023

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