Efficient production of icariin and baohuoside I from Epimedium Folium flavonoids by fungal α-l-rhamnosidase hydrolysing regioselectively the terminal rhamnose of epimedin C

Biotechnology for Biofuels and Bioproducts

Research

Shiping Cao¹  Guangwei Wu¹  Linguo Zhao¹  Changning Lu¹  Qi Li¹  Shanshan Zhang¹ 

[1] Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, 210037, Nanjing, China;College of Chemical Engineering, Nanjing Forestry University, 159 Long Pan Road, 210037, Nanjing, China;

关键词: α-;    Icariin;    Baohuoside I;    Epimedium Folium;    Biotransformation;   

DOI  :  10.1186/s13068-023-02348-6

 received in 2022-12-08, accepted in 2023-05-23,  发布年份 2023

来源: Springer

【 摘 要 】

Industrial application of icariin and baohuoside I has been hindered by the short supply to a great extent. In this work, a novel GH78 α-l-rhamnosidase AmRha catalyzed the bioconversion of low-value epimedin C in crude Epimedium Folium flavonoids (EFs) to icariin and baohuoside I was developed. Firstly, the high-level expression of AmRha in Komagataella phaffii GS115 attained an enzyme activity of 571.04 U/mL. The purified recombinant AmRha could hydrolyze α-1,2-rhamnoside bond between two rhamnoses (α-Rha(2 → 1)α-Rha) in epimedin C to produce icariin with a molar conversion rate of 92.3%, in vitro. Furtherly, the biotransformation of epimedin C to icariin by the recombinant Komagataella phaffii GS115 cells was also investigated, which elevated the EFs concentration by fivefold. In addition, biotransformation of epimedins A-C and icariin in the raw EFs to baohuoside I was fulfilled by a collaboration of AmRha and β-glucosidase/β-xylosidase Dth3. The results obtained here provide a new insight into the preparation of high-value products icariin and baohuoside I from cheap raw EFs.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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