Selenoproteins synergistically protect porcine skeletal muscle from oxidative damage via relieving mitochondrial dysfunction and endoplasmic reticulum stress

Journal of Animal Science and Biotechnology

Research

Bo Kang¹  Lianqiang Che²  Longqiong Wang²  Gang Jia²  Jingyi Cai²  Xiaoling Chen²  Guangmang Liu²  Jinzhong Jing²  Ying He²  Jiayong Tang²  Hua Zhao²  Gang Tian²  Yan Liu² 

[1] College of Animal Science and Technology, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China;Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, of China Ministry of Agriculture and Rural Affairs, of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China;

关键词: Dietary oxidative stress;    Endoplasmic reticulum stress;    Growth retardation;    Mitochondrial dysfunction;    Selenoproteins;    Skeletal muscle;   

DOI  :  10.1186/s40104-023-00877-6

 received in 2022-12-12, accepted in 2023-04-05,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe skeletal muscle of pigs is vulnerable to oxidative damage, resulting in growth retardation. Selenoproteins are important components of antioxidant systems for animals, which are generally regulated by dietary selenium (Se) level. Here, we developed the dietary oxidative stress (DOS)-inducing pig model to investigate the protective effects of selenoproteins on DOS-induced skeletal muscle growth retardation.ResultsDietary oxidative stress caused porcine skeletal muscle oxidative damage and growth retardation, which is accompanied by mitochondrial dysfunction, endoplasmic reticulum (ER) stress, and protein and lipid metabolism disorders. Supplementation with Se (0.3, 0.6 or 0.9 mg Se/kg) in form of hydroxy selenomethionine (OH-SeMet) linearly increased muscular Se deposition and exhibited protective effects via regulating the expression of selenotranscriptome and key selenoproteins, which was mainly reflected in lower ROS levels and higher antioxidant capacity in skeletal muscle, and the mitigation of mitochondrial dysfunction and ER stress. What's more, selenoproteins inhibited DOS induced protein and lipid degradation and improved protein and lipid biosynthesis via regulating AKT/mTOR/S6K1 and AMPK/SREBP-1 signalling pathways in skeletal muscle. However, several parameters such as the activity of GSH-Px and T-SOD, the protein abundance of JNK2, CLPP, SELENOS and SELENOF did not show dose-dependent changes. Notably, several key selenoproteins such as MSRB1, SELENOW, SELENOM, SELENON and SELENOS play the unique roles during this protection.ConclusionsIncreased expression of selenoproteins by dietary OH-SeMet could synergistically alleviate mitochondrial dysfunction and ER stress, recover protein and lipid biosynthesis, thus alleviate skeletal muscle growth retardation. Our study provides preventive measure for OS-dependent skeletal muscle retardation in livestock husbandry.

【 授权许可】

CC BY   
© The Author(s) 2023

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