Genetic mapping and identification of Rht8-B1 that regulates plant height in wheat

BMC Plant Biology

Research

Huijun Guo¹  Jiayu Gu¹  Shirong Zhao¹  Yuping Ding¹  Yuting Li¹  Hongchun Xiong¹  Linshu Zhao¹  Luxiang Liu¹  Yongdun Xie¹  Xuejun Li²  Meiyu Fu³  Chunyun Zhou³ 

[1] Institute of Crop Sciences, National Engineering Laboratory for Crop Molecular Breeding, Chinese Academy of Agricultural Sciences, National Center of Space Mutagenesis for Crop Improvement, Beijing, China;State Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, 712100, Yangling, Shaanxi, China;State Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, 712100, Yangling, Shaanxi, China;Institute of Crop Sciences, National Engineering Laboratory for Crop Molecular Breeding, Chinese Academy of Agricultural Sciences, National Center of Space Mutagenesis for Crop Improvement, Beijing, China;

关键词: Wheat;    Plant height;    Rht8-B1;    QTL mapping;    Spike compactness;   

DOI  :  10.1186/s12870-023-04343-3

 received in 2023-02-14, accepted in 2023-06-11,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundPlant height (PH) and spike compactness (SC) are important agronomic traits that affect yield improvement in wheat crops. The identification of the loci or genes responsible for these traits is thus of great importance for marker-assisted selection in wheat breeding.ResultsIn this study, we used a recombinant inbred line (RIL) population with 139 lines derived from a cross between the mutant Rht8-2 and the local wheat variety NongDa5181 (ND5181) to construct a high-density genetic linkage map by applying the Wheat 40 K Panel. We identified seven stable QTLs for PH (three) and SC (four) in two environments using the RIL population, and found that Rht8-B1 is the causal gene of qPH2B.1 by further genetic mapping, gene cloning and gene editing analyses. Our results also showed that two natural variants from GC to TT in the coding region of Rht8-B1 resulted in an amino acid change from G (ND5181) to V (Rht8-2) at the 175th position, reducing PH by 3.6%~6.2% in the RIL population. Moreover, gene editing analysis suggested that the height of T2 generation in Rht8-B1 edited plants was reduced by 5.6%, and that the impact of Rht8-B1 on PH was significantly lower than Rht8-D1. Additionally, analysis of the distribution of Rht8-B1 in various wheat resources suggested that the Rht8-B1b allele has not been widely utilized in modern wheat breeding.ConclusionsThe combination of Rht8-B1b with other favorable Rht genes might be an alternative approach for developing lodging-resistant crops. Our study provides important information for marker-assisted selection in wheat breeding.

【 授权许可】

CC BY   
© The Author(s) 2023

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