A streptavidin–biotin system combined with magnetic actuators for remote neuronal guidance

Journal of Biological Engineering

Research

Amos Sharoni¹  Lidor Geri¹  Ganit Indech¹  Dafna Rivka Levenberg²  Orit Shefi²  Eli Varon²  Tal Ben Uliel² 

[1] Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, 5290002, Ramat Gan, Israel;Department of Physics, Bar-Ilan University, 5290002, Ramat Gan, Israel;Faculty of Engineering, Bar-Ilan University, 5290002, Ramat Gan, Israel;Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, 5290002, Ramat Gan, Israel;Gonda Multidisciplinary Brain Research Center, Bar-Ilan University, 5290002, Ramat Gan, Israel;

关键词: Magnetic manipulation;    Neuronal organization;    Streptavidin–biotin;    Magnetic particles;   

DOI  :  10.1186/s13036-023-00359-3

 received in 2022-12-20, accepted in 2023-05-30,  发布年份 2023

来源: Springer

【 摘 要 】

The ability to control neuronal mobility and organization is of great importance in developing neuronal interfaces and novel therapeutic approaches. An emerging promising method is the manipulation of neuronal cells from afar via magnetic forces. Nevertheless, using magnetic iron oxide nanoparticles as internal actuators may lead to biotoxicity, adverse influence on intracellular processes, and thus requires prerequisite considerations for therapeutic approaches. Magnetizing the cells via the incorporation of magnetic particles that can be applied extracellularly is advantageous. Herein, we have developed a magnetic system based on streptavidin–biotin interaction to decorate cellular membrane with magnetic elements. In this model, superparamagnetic microparticles, coated with streptavidin, were specifically bound to biotinylated PC12 cells. We demonstrated that cell movement can be directed remotely by the forces produced by pre-designed magnetic fields. First, using time lapse imaging, we analyzed the kinetics of cell migration towards the higher flux zone. Next, to form organized networks of cells we designed and fabricated micro-patterned magnetic devices. The fabricated devices were composed of a variety of ferromagnetic shapes, sputter-deposited onto glass substrates. Cells that were conjugated to the magnetic particles were plated atop the micro-patterned substrates, attracted to the magnetic actuators and became fixed onto the magnetic patterns. In all, our study presents a novel system based on a well-known molecular technology combined with nanotechnology that may well lead to the expansion of implantable magnetic actuators to organize and direct cellular growth.

【 授权许可】

CC BY   
© The Author(s) 2023

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