Notch mediates the glycolytic switch via PI3K/Akt signaling to support embryonic development

Cellular & Molecular Biology Letters

Research Letter

Wence Wang¹  Xuyang Wang¹  Wenqi Liang¹  Yongwen Zhu¹  Lin Yang¹  Heng Wang¹  Yuchun Zhan¹ 

[1] Guangdong Provincial Key Laboratory of Animal Nutrition and Regulation, College of Animal Science, South China Agricultural University, 510000, Guangzhou, China;

关键词: Notch signaling;    Glycolysis;    PI3K/Akt;    Avian embryonic development;   

DOI  :  10.1186/s11658-023-00459-4

 received in 2023-01-14, accepted in 2023-05-05,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundEnergy metabolism disorder or insufficient energy supply during incubation will affect the development and survival of avian embryos. Especially, β-oxidation could not provide the continuous necessary energy for avian embryonic development due to the increasing energy demand under hypoxic conditions during the mid–late embryonic stages. The role and mechanism of hypoxic glycolysis replacing β-oxidation as the main source of energy supply for avian embryonic development in the mid–late stages is unclear.ResultsHere, we found that in ovo injection with glycolysis inhibitor or γ-secretase inhibitor both decreased the hepatic glycolysis level and impaired goose embryonic development. Intriguingly, the blockade of Notch signaling is also accompanied by the inhibition of PI3K/Akt signaling in the embryonic primary hepatocytes and embryonic liver. Notably, the decreased glycolysis and impaired embryonic growth induced by the blockade of Notch signaling were restored by activation of PI3K/Akt signaling.ConclusionsNotch signaling regulates a key glycolytic switch in a PI3K/Akt-dependent manner to supply energy for avian embryonic growth. Our study is the first to demonstrate the role of Notch signaling-induced glycolytic switching in embryonic development, and presents new insight into the energy supply patterns in embryogenesis under hypoxic conditions. In addition, it may also provide a natural hypoxia model for developmental biology studies such as immunology, genetics, virology, cancer, etc.Graphical Abstract

【 授权许可】

CC BY   
© The Author(s) 2023

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