Microbiota members from body sites of dairy cows are largely shared within individual hosts throughout lactation but sharing is limited in the herd

Animal Microbiome

Research

Fabienne Constant¹  Laurent X. Nouvel²  Christine Citti²  Yves Le Loir³  Lucie Rault³  Sergine Even³  Sarah Barbey⁴  Frederic Launay⁴  Pierre Germon⁵  Emmanuelle Helloin⁵  Diego P. Morgavi⁶  Rachel Lefebvre⁷  Olivier Rué⁸  Mahendra Mariadassou⁹  Sophie Schbath⁹  Olivier Sandra^1,10 

[1] Ecole Nationale Vétérinaire d’Alfort, Université Paris-Saclay, UVSQ, INRAE, BREED, Maisons-Alfort, France;IHAP, Université de Toulouse, INRAE, ENVT, Toulouse, France;INRAE, Institut Agro, UMR1253 STLO, Rennes, France;INRAE, UE326 Unité Expérimentale du Pin, Gouffern en Auge, France;INRAE, Université de Tours, UMR ISP, 37380, Nouzilly, France;Université Clermont Auvergne, INRAE, VetAgro Sup, UMR Herbivores, Saint-Genes-Champanelle, France;Université Paris-Saclay, INRAE, AgroParisTech, GABI, Jouy‐en‐Josas, France;Université Paris-Saclay, INRAE, BioinfOmics, MIGALE Bioinformatics Facility, Jouy-en-Josas, France;Université Paris-Saclay, INRAE, MaIAGE, Jouy-en-Josas, France;Université Paris-Saclay, UVSQ, INRAE, BREED, Jouy-en-Josas, France;

关键词: Bovine holobiont;    Dairy cow microbiota;    Vaginal microbiota;    Milk microbiota;    Nasal microbiota;    Oral microbiota;    Mastitis susceptibility;    Microbial sharing;   

DOI  :  10.1186/s42523-023-00252-w

 received in 2022-09-22, accepted in 2023-06-06,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundHost-associated microbes are major determinants of the host phenotypes. In the present study, we used dairy cows with different scores of susceptibility to mastitis with the aim to explore the relationships between microbiota composition and different factors in various body sites throughout lactation as well as the intra- and inter-animal microbial sharing.ResultsMicrobiotas from the mouth, nose, vagina and milk of 45 lactating dairy cows were characterized by metataxonomics at four time points during the first lactation, from 1-week pre-partum to 7 months post-partum. Each site harbored a specific community that changed with time, likely reflecting physiological changes in the transition period and changes in diet and housing. Importantly, we found a significant number of microbes shared among different anatomical sites within each animal. This was between nearby anatomic sites, with up to 32% of the total number of Amplicon Sequence Variants (ASVs) of the oral microbiota shared with the nasal microbiota but also between distant ones (e.g. milk with nasal and vaginal microbiotas). In contrast, the share of microbes between animals was limited (< 7% of ASVs shared by more than 50% of the herd for a given site and time point). The latter widely shared ASVs were mainly found in the oral and nasal microbiotas. These results thus indicate that despite a common environment and diet, each animal hosted a specific set of bacteria, supporting a tight interplay between each animal and its microbiota. The score of susceptibility to mastitis was slightly but significantly related to the microbiota associated to milk suggesting a link between host genetics and microbiota.ConclusionsThis work highlights an important sharing of microbes between relevant microbiotas involved in health and production at the animal level, whereas the presence of common microbes was limited between animals of the herd. This suggests a host regulation of body-associated microbiotas that seems to be differently expressed depending on the body site, as suggested by changes in the milk microbiota that were associated to genotypes of susceptibility to mastitis.

【 授权许可】

CC BY   
© The Author(s) 2023

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