Rapid activation of hematopoietic stem cells

Stem Cell Research & Therapy

Short Report

Omri Sharabi¹  Roi Gazit¹  Erez Elfassy¹  Leonid Olender¹  Roshina Thapa¹ 

[1] The Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, National Institute for Biotechnology in the Negev, The Ben-Gurion University of the Negev, 84105, Beer Sheva, Israel;

关键词: Hematopoietic stem cells;    HSC;    Activation markers;    Rapid activation;    CD69;    CD317;    BST2;    Ki67;    Differentiation;    Proliferation;   

DOI  :  10.1186/s13287-023-03377-6

 received in 2022-11-07, accepted in 2023-05-11,  发布年份 2023

来源: Springer

【 摘 要 】

Adult hematopoietic stem cells (HSCs) in the bone marrow (BM) are quiescent. Following perturbations, such as blood loss or infection, HSCs may undergo activation. Surprisingly, little is known about the earliest stages of HSCs activation. We utilize surface markers of HSCs activation, CD69 and CD317, revealing a response as early as 2 h after stimulation. The dynamic expression of HSCs activation markers varies between viral-like (poly-Inosinic-poly-Cytidylic) or bacterial-like (Lipopolysaccharide) immune stimuli. We further quantify dose response, revealing a low threshold, and similar sensitivity of HSCs and progenitors in the BM. Finally, we find a positive correlation between the expression of surface activation markers and early exit from quiescence. Our data show that the response of adult stem cells to immune stimulation is rapid and sensitive, rapidly leading HSCs out of quiescence.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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