The synbiotic mixture of lactulose and Bacillus coagulans protects intestinal barrier dysfunction and apoptosis in weaned piglets challenged with lipopolysaccharide

Journal of Animal Science and Biotechnology

Research

Weijiang Zheng¹  Yunnan Yang¹  Zuyan Zhao¹  Wen Yao²  Liren Ding³ 

[1]College of Animal Science and Technology, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, China

[2]College of Animal Science and Technology, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, China

[3]Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, 210095, Nanjing, Jiangsu, China

[4]National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, China

关键词: Apoptosis;    Bacillus coagulans;    Intestinal barrier function;    Lactulose;    LPS;    Piglets;    Synbiotic;   

DOI  :  10.1186/s40104-023-00882-9

 received in 2022-12-19, accepted in 2023-04-10,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundLactulose as an effective prebiotic protects intestinal mucosal injury. Bacillus coagulans is widely used in feed additives because of its ability to promote intestinal health. Our previous study suggests that the combination of lactulose and Bacillus coagulans may be a good candidate as alternative for antibiotic growth promoters. However, the in vivo effects of lactulose and Bacilluscoagulans on growth and intestinal health under immune challenge in piglets remains unclear. The objective of this study is to explore the protective effects of synbiotic containing lactulose and Bacillus coagulans on the intestinal mucosal injury and barrier dysfunction under immune challenge in weaned piglets.MethodsTwenty four weaned piglets were assigned to 4 groups. Piglets in the CON-saline and LPS-LPS group were fed the basal diet, while others were fed either with chlortetracycline (CTC) or synbiotic mixture of lactulose and Bacillus coagulans for 32 d before injection of saline or lipopolysaccharide (LPS). Piglets were sacrificed 4 h after LPS injection to collect samples to determine intestinal morphology, integrity and barrier functions as well as relative genes and proteins.ResultsOur data showed that no differences were observed in the growth performance of the four test groups. LPS injection induced higher serum diamine oxidase activities, D-lactic acid levels, and endotoxin status, lower villus height and ratio of villus height to crypt depth, greater mRNA and lower protein expression related tight junction in both jejunum and ileum. In addition, a higher apoptosis index, and protein expression of Bax and caspase-3 were also observed in the LPS challenge group. Interestingly, dietary synbiotic mixture with lactulose and Bacillus coagulans protected against LPS-induced intestinal damage, barrier dysfunction and higher apoptosis as well as CTC.ConclusionsOur data suggest that dietary supplementation of synbiotic mixture with lactulose and Bacillus coagulans showed resilience to LPS-induced intestinal morphological damage, barrier dysfunction and aggressive apoptosis in piglets as well as the protective effects of CTC. These results indicate that synbiotic mixture of lactulose and Bacillus coagulans showed beneficial effects on performance and resilience to acute immune stress in weaned piglets.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

Fig. 1

40517_2023_259_Article_IEq19.gif

13011_2023_540_Article_IEq5.gif

13011_2023_540_Article_IEq3.gif

41116_2023_37_Article_IEq285.gif