Early stage evaluation of cancer stem cells using platinum nanoparticles/CD133+ enhanced nanobiocomposite

Cancer Nanotechnology

Research

Ibrahim Isildak¹  Farhad Bani²  Solmaz Sadi²  Soodabeh Davaran²  Mohammad-Reza Rashidi³  Balal Khalilzadeh⁴  Mahdi Mahdipour⁴  Fatemeh Sokouti Nasimi⁴ 

[1] Department of Bioengineering, Faculty of Chemistry-Metallurgy, Yildiz Technical University, 34220, Istanbul, Turkey;Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, 516661-4733, Tabriz, Iran;Department of Medicinal Chemistry, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran;Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;

关键词: Cancer stem cells;    CD;    Platinum nanoparticles;    Silica nanocomposite;    Biosensor;    Colorectal cancer;   

DOI  :  10.1186/s12645-023-00208-4

 received in 2023-04-02, accepted in 2023-05-02,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundCancer stem cells (CSCs) are of great diagnostic importance due to their involvement in tumorigenesis, therapeutic resistance, metastasis and relapse.MethodIn this work, a sensitive electrochemical cytosensor was successfully established to detect HT-29 colorectal cancer stem cells based on a nanocomposite composed of mesoporous silica nanoparticles (MSNs) and platinum nanoparticles (PtNPs) using a simple and fast electrodeposition technique on a glassy carbon electrode (GCE).ResultsAccording to SEM images, the PtNPs nanoparticles formed on the MSNs substrate are about 100 nm. As expected, high-rate porosity, increased surface-to-volume ratio, provides appropriate local electron transfer rate and suitable platform for the efficient formation of PtNPs. These features allow direct and stable binding of biotinylated monoclonal antibody of CD133 to streptavidin (Strep) and the subsequent availability of active sites for CSCs identification. Differential pulse voltammetry (DPV) results show that close interaction of CD133+ cells with monoclonal antibodies reduces charge transfer and electrical current, as confirmed by square wave voltammogram (SWV). Based on the recorded current versus number of CSCs, we noted that our developed system can sense CSCs from 5 to 20 cells/5 μL.ConclusionsAs a proof of concept, the designed nanobiocomposite was able to specifically detect CD133+ cells compared to whole HT-29 cells before magnetic activated cell sorting (MACS) process.

【 授权许可】

CC BY   
© The Author(s) 2023

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