Light in the box—photobiological examination chamber with light trap ventilation system for studying fungal surface cultures illustrated with Metarhizium brunneum and Beauveria brongniartii

Fungal Biology and Biotechnology

Research

Lucia Colleselli¹  Maria Zottele²  Hermann Strasser²  Pamela Vrabl²  Christoph Walter Schinagl³  Laura Mayerhofer⁴  Bianka Siewert⁴ 

[1] Department of Biotechnology & Food Engineering, MCI-The Entrepreneurial School, Maximilianstraße 2, A-6020, Innsbruck, Austria;Institute of Microbiology, University of Innsbruck, Technikerstraße 25, 6020, Innsbruck, Austria;Institute of Microbiology, University of Innsbruck, Technikerstraße 25, 6020, Innsbruck, Austria;Department of Biotechnology & Food Engineering, MCI-The Entrepreneurial School, Maximilianstraße 2, A-6020, Innsbruck, Austria;Institute of Pharmacy/Pharmacognosy, Center for Molecular Biosciences Innsbruck (CMBI), Center for Chemistry and Biomedicine, University of Innsbruck, Innrain 80-82, A-6020, Innsbruck, Austria;

关键词: Entomopathogenic fungi;    Fungal photobiology;    Light box incubator;    Photo-illumination setup;    Visible light;    LED-technique;    Surface culture;    Extrolite;    Conidia;    Morphology;   

DOI  :  10.1186/s40694-023-00159-w

 received in 2023-02-10, accepted in 2023-05-11,  发布年份 2023

来源: Springer

【 摘 要 】

Due to their versatile way of life as saprophytes, endophytes, and entomopathogens, fungi of the genera Metarhizium and Beauveria are exposed to varying illumination conditions in their natural habitats, which makes a thorough adaptation to light very likely. While the few available studies for these genera support this assumption, research in this field is still in its infancy and the data material restricted to only a few fungal species. Thus, the aim of this work was to explore how light influences growth, conidial production and secondary metabolite formation of two industrial relevant strains of M. brunneum (MA 43, formerly M. anisopliae var. anisopliae BIPESCO 5/F52) and B. brongniartii (BIPESCO 2). To achieve this, we constructed an easily adjustable illumination device for highly standardized photophysiological studies of fungi on Petri dishes, the so-called LIGHT BOX. With the aid of this device, M. brunneum and B. brongniartii were grown on S4G or S2G agar at 25 °C for 14 days either in complete darkness or under constant illumination with red light (λpeak = 635 nm), green light (λpeak = 519 nm) or blue light (λpeak = 452 nm). In addition, for each wavelength the effect of different illumination intensities was tested, i.e., intensities of red light ranging from 22.1 ± 0.1 to 136.5 ± 0.3 µW cm-2, green light from 16.5 ± 0.1 to 96.2 ± 0.1 µW cm-2, and blue light from 56.1 ± 0.2 to 188.9 ± 0.6 µW cm-2. Both fungi strongly responded in terms of growth, conidial production, pigmentation and morphology to changes in the wavelength and irradiation intensity. The wavelength-dependent production of the well-known secondary metabolite oosporein which is secreted by the genus Beauveria in particular, was also increased under green and blue light exposure. The established LIGHT BOX system allows not only to optimize conidial production yields with these biotechnologically relevant fungi, but also allows the photobiological exploration of other fungi.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

Fig. 3

41116_2023_36_Article_IEq399.gif

12938_2023_1111_Article_IEq24.gif

41116_2023_36_Article_IEq375.gif

41116_2023_36_Article_IEq353.gif

41116_2023_36_Article_IEq346.gif

40517_2023_256_Article_IEq256.gif

41116_2023_36_Article_IEq327.gif