Myogenic differentiation of human myoblasts and Mesenchymal stromal cells under GDF11 on NPoly-ɛ-caprolactone-collagen I-Polyethylene-nanofibers

BMC Molecular and Cell Biology

Research

Justus P. Beier¹  Aijia Cai²  Raymund E. Horch²  Andreas Arkudas²  Zeng-Ming Zheng²  Paul Schneider²  Volker Weisbach³  Dirk W. Schubert⁴  Marcus Himmler⁴ 

[1] Department of Plastic Surgery, Hand Surgery - Burn Center, University Hospital RWTH Aachen, 52074, Aachen, Germany;Department of Plastic and Hand Surgery, Laboratory for Tissue Engineering and Regenerative Medicine, University Hospital of Erlangen, Friedrich-Alexander University of Erlangen-Nürnberg (FAU), 91054, Erlangen, Germany;Department of Transfusion Medicine, University Hospital of Erlangen, Friedrich-Alexander- University Erlangen-Nürnberg (FAU), 91054, Erlangen, Germany;Institute of Polymer Materials, Department of Materials Science and Engineering, University of Erlangen-Nürnberg (FAU), 91058, Erlangen, Germany;

关键词: Myogenic differentiation;    Nanoscaffolds;    Electrospun composite nanofibers;    poly-ε-caprolacton (PCL);    Collagen;    Polyethylene oxide (PEO);    ADSC;    Myoblasts;    GDF11;    Myostatin;   

DOI  :  10.1186/s12860-023-00478-1

 received in 2023-02-19, accepted in 2023-04-27,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundFor the purpose of skeletal muscle engineering, primary myoblasts (Mb) and adipogenic mesenchymal stem cells (ADSC) can be co-cultured and myogenically differentiated. Electrospun composite nanofiber scaffolds represent suitable matrices for tissue engineering of skeletal muscle, combining both biocompatibility and stability Although growth differentiation factor 11 (GDF11) has been proposed as a rejuvenating circulating factor, restoring skeletal muscle function in aging mice, some studies have also described a harming effect of GDF11. Therefore, the aim of the study was to analyze the effect of GDF11 on co-cultures of Mb and ADSC on poly-ε-caprolactone (PCL)-collagen I-polyethylene oxide (PEO)-nanofibers.ResultsHuman Mb were co-cultured with ADSC two-dimensionally (2D) as monolayers or three-dimensionally (3D) on aligned PCL-collagen I-PEO-nanofibers. Differentiation media were either serum-free with or without GDF11, or serum containing as in a conventional differentiation medium. Cell viability was higher after conventional myogenic differentiation compared to serum-free and serum-free + GDF11 differentiation as was creatine kinase activity. Immunofluorescence staining showed myosine heavy chain expression in all groups after 28 days of differentiation without any clear evidence of more or less pronounced expression in either group. Gene expression of myosine heavy chain (MYH2) increased after serum-free + GDF11 stimulation compared to serum-free stimulation alone.ConclusionsThis is the first study analyzing the effect of GDF11 on myogenic differentiation of Mb and ADSC co-cultures under serum-free conditions. The results of this study show that PCL-collagen I-PEO-nanofibers represent a suitable matrix for 3D myogenic differentiation of Mb and ADSC. In this context, GDF11 seems to promote myogenic differentiation of Mb and ADSC co-cultures compared to serum-free differentiation without any evidence of a harming effect.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

41116_2023_36_Article_IEq714.gif

41116_2023_36_Article_IEq631.gif

41116_2023_36_Article_IEq483.gif

41116_2023_36_Article_IEq431.gif

41116_2023_36_Article_IEq430.gif

41116_2023_36_Article_IEq408.gif

41116_2023_36_Article_IEq393.gif

41116_2023_36_Article_IEq385.gif

41116_2023_36_Article_IEq377.gif