Genetic Mapping of the Root Mycobiota in Rice and its Role in Drought Tolerance

Rice

Research

Willem Kruijer¹  Dennis E. te Beest¹  Nathan Vannier²  Philippe Vandenkoornhuyse²  Niteen N. Kadam³  S. V. Krishna Jagadish⁴  Carolien Ruyter-Spira⁵  Beatriz Andreo-Jimenez⁶  Harro J. Bouwmeester⁷  Giovanni Melandri⁸  Gerard van der Linden⁹ 

[1] Biometris, Wageningen University and Research, Wageningen, The Netherlands;EcoBio, CNRS, Université de Rennes I, Rennes, France;International Rice Research Institute, Los Baños, Laguna, Philippines;Centre for Crop Systems Analysis, Wageningen University and Research, Wageningen, The Netherlands;International Rice Research Institute, Los Baños, Laguna, Philippines;Kansas State University, 66506, Manhattan, KS, USA;Laboratory of Plant Physiology, Wageningen University and Research, Wageningen, The Netherlands;Laboratory of Plant Physiology, Wageningen University and Research, Wageningen, The Netherlands;Biointeractions and Plant Health, Wageningen University and Research, Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands;Laboratory of Plant Physiology, Wageningen University and Research, Wageningen, The Netherlands;Plant Hormone Biology Group, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, The Netherlands;Laboratory of Plant Physiology, Wageningen University and Research, Wageningen, The Netherlands;School of Plant Sciences, University of Arizona, Tucson, USA;Plant Breeding, Wageningen University and Research, Wageningen, Netherlands;

关键词: Drought;    Fungi;    Root mycobiota;    GWAS;    Oryza sativa;    Roots;   

DOI  :  10.1186/s12284-023-00641-4

 received in 2023-02-05, accepted in 2023-05-11,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundRice is the second most produced crop worldwide, but is highly susceptible to drought. Micro-organisms can potentially alleviate the effects of drought. The aim of the present study was to unravel the genetic factors involved in the rice-microbe interaction, and whether genetics play a role in rice drought tolerance. For this purpose, the composition of the root mycobiota was characterized in 296 rice accessions (Oryza sativa L. subsp. indica) under control and drought conditions. Genome wide association mapping (GWAS) resulted in the identification of ten significant (LOD > 4) single nucleotide polymorphisms (SNPs) associated with six root-associated fungi: Ceratosphaeria spp., Cladosporium spp., Boudiera spp., Chaetomium spp., and with a few fungi from the Rhizophydiales order. Four SNPs associated with fungi-mediated drought tolerance were also found. Genes located around those SNPs, such as a DEFENSIN-LIKE (DEFL) protein, EXOCYST TETHERING COMPLEX (EXO70), RAPID ALKALINIZATION FACTOR-LIKE (RALFL) protein, peroxidase and xylosyltransferase, have been shown to be involved in pathogen defense, abiotic stress responses and cell wall remodeling processes. Our study shows that rice genetics affects the recruitment of fungi, and that some fungi affect yield under drought. We identified candidate target genes for breeding to improve rice-fungal interactions and hence drought tolerance.

【 授权许可】

CC BY   
© The Author(s) 2023

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