Association of a high-fat diet with I-FABP as a biomarker of intestinal barrier dysfunction driven by metabolic changes in Wistar rats

Lipids in Health and Disease

Research

Jazib Hussain¹  Humaira Muzaffar²  Haseeb Anwar²  Aisha Mahmood³  Faqir Muhammad⁴  Junaid Ali Khan⁵  Jawad Aslam⁶  Muhammad Naeem Faisal⁶ 

[1] Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark;Department of Physiology, Government College University, 38040, Faisalabad, Pakistan;Department of Physiology, The Islamia University of Bahawalpur, 63100, Bahawalpur, Pakistan;Faculty of Veterinary Science, Bahaudin Zakariya University, Multan, Pakistan;Faculty of Veterinary and Animal Sciences, Muhammad Nawaz Shareef University of Agriculture, Multan, Pakistan;Institute of Physiology and Pharmacology, University of Agriculture, 38040, Faisalabad, Pakistan;

关键词: High-fat diet;    Metabolic disorders;    Obesity;    Dyslipidemia;    Intestinal fatty acid binding protein;    Insulin and leptin resistance;   

DOI  :  10.1186/s12944-023-01837-9

 received in 2022-05-16, accepted in 2023-05-23,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe epithelial lining of the gut expresses intestinal fatty-acid binding proteins (I-FABPs), which increase in circulation and in plasma concentration during intestinal damage. From the perspective of obesity, the consumption of a diet rich in fat causes a disruption in the integrity of the gut barrier and an increase in its permeability.HypothesisThere is an association between the expression of I-FABP in the gut and various metabolic changes induced by a high-fat (HF) diet.MethodsWistar albino rats (n = 90) were divided into three groups (n = 30 per group), viz. One control and two HF diet groups (15 and 30%, respectively) were maintained for 6 weeks. Blood samples were thus collected to evaluate the lipid profile, blood glucose level and other biochemical tests. Tissue sampling was conducted to perform fat staining and immunohistochemistry.ResultsHF diet-fed rats developed adiposity, insulin resistance, leptin resistance, dyslipidemia, and increased expression of I-FABP in the small intestine compared to the control group. Increased I-FABP expression in the ileal region of the intestine is correlated significantly with higher fat contents in the diet, indicating that higher I-FABP expression occurs due to increased demand of enterocytes to transport lipids, leading to metabolic alterations.ConclusionIn summary, there is an association between the expression of I-FABP and HF diet-induced metabolic alterations, indicating that I-FABP can be used as a biomarker for intestinal barrier dysfunction.

【 授权许可】

CC BY   
© The Author(s) 2023

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