Genome-wide expression analysis reveals different heat shock responses in indigenous (Bos indicus) and crossbred (Bos indicus X Bos taurus) cattle

Genes and Environment

Research

Aditya Prasad Sahoo¹  Bishnu Prasad Mishra²  Gundallahalli B. Manjunathareddy³  Gyanendra Singh⁴  Sujoy K. Dhara⁵  Praveen K. Gupta⁵  Triveni Dutt⁵  Basavaraj Sajjanar⁵  Mohd Tanzeel Aalam⁵  Owais Khan⁵  Gunturu Narasimha Tanuj⁵  Ravi Kumar Gandham⁵ 

[1] ICAR- Directorate of Foot and Mouth Disease, 752050, Bhubaneswar, Odisha, India;ICAR-National Bureau of Animal Genetic Resources, 132001, Karnal, Haryana, India;ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, 560064, Yelahanka, Karnataka, India;Physiology and Climatology Division, ICAR-Indian Veterinary Research Institute, Izatnagar, 243122, Bareilly, Uttar Pradesh, India;Veterinary Biotechnology Division, ICAR-Indian Veterinary Research Institute, Izatnagar, 243122, Bareilly, Uttar Pradesh, India;

关键词: Heat stress;    Dairy cattle;    Immune response;    Gene expression;   

DOI  :  10.1186/s41021-023-00271-8

 received in 2022-11-06, accepted in 2023-04-03,  发布年份 2023

来源: Springer

【 摘 要 】

Environmental heat stress in dairy cattle leads to poor health, reduced milk production and decreased reproductive efficiency. Multiple genes interact and coordinate the response to overcome the impact of heat stress. The present study identified heat shock regulated genes in the peripheral blood mononuclear cells (PBMC). Genome-wide expression patterns for cellular stress response were compared between two genetically distinct groups of cattle viz., Hariana (B. indicus) and Vrindavani (B. indicus X B. taurus). In addition to major heat shock response genes, oxidative stress and immune response genes were also found to be affected by heat stress. Heat shock proteins such as HSPH1, HSPB8, FKB4, DNAJ4 and SERPINH1 were up-regulated at higher fold change in Vrindavani compared to Hariana cattle. The oxidative stress response genes (HMOX1, BNIP3, RHOB and VEGFA) and immune response genes (FSOB, GADD45B and JUN) were up-regulated in Vrindavani whereas the same were down-regulated in Hariana cattle. The enrichment analysis of dysregulated genes revealed the biological functions and signaling pathways that were affected by heat stress. Overall, these results show distinct cellular responses to heat stress in two different genetic groups of cattle. This also highlight the long-term adaptation of B. indicus (Hariana) to tropical climate as compared to the crossbred (Vrindavani) with mixed genetic makeup (B. indicus X B. taurus).

【 授权许可】

CC BY   
© The Author(s) 2023

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