Evaluation of pathotype marker genes in Streptococcus suis isolated from human and clinically healthy swine in Thailand

BMC Microbiology

Research

Nuchsupha Sunthamala¹  Kulsatri Sittichottumrong²  Nichari Bamphensin²  Anusak Kerdsin²  Parichart Boueroy²  Peechanika Chopjitt²  Ratchadaporn Ungcharoen²  Rujirat Hatrongjit³  Marcelo Gottschalk⁴ 

[1] Department of Biology, Faculty of Science, Mahasarakham University, 44150, Mahasarakham, Thailand;Faculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, 47000, Sakon Nakhon, Thailand;Faculty of Science and Engineering, Kasetsart University Chalermphrakiat Sakon Nakhon, Province Campus, 47000, Sakon Nakhon, Thailand;Groupe de recherche sur les maladies infectieuses en production animale (GREMIP), The Swine and Poultry Infectious Diseases Research Centre (CRIPA), Faculty of Veterinary Medicine, University of Montreal, Québec, Canada;

关键词: Streptococcus suis;    Serotype;    Sequence type;    Pathotype;    Multiplex PCR;   

DOI  :  10.1186/s12866-023-02888-9

 received in 2023-03-14, accepted in 2023-05-09,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundStreptococcus suis is a zoonotic pathogen that causes substantial economic losses in the pig industry and contributes to human infections worldwide, especially in Southeast Asia. Recently, a multiplex polymerase chain reaction (PCR) process was developed to distinguish disease-associated and non-disease-associated pathotypes of S. suis European strains. Herein, we evaluated the ability of this multiplex PCR approach to distinguish pathotypes of S. suis in Thailand.ResultsThis study was conducted on 278 human S. suis isolates and 173 clinically healthy pig S. suis isolates. PCR identified 99.3% of disease-associated strains in the human isolates and 11.6% of non-disease-associated strains in the clinically healthy pig isolates. Of the clinically healthy pig S. suis isolates, 71.1% were classified as disease-associated. We also detected undetermined pathotype forms in humans (0.7%) and pigs (17.3%). The PCR assay classified the disease-associated isolates into four types. Statistical analysis revealed that human S. suis clonal complex (CC) 1 isolates were significantly associated with the disease-associated type I, whereas CC104 and CC25 were significantly associated with the disease-associated type IV.ConclusionMultiplex PCR cannot differentiate non-disease-associated from disease-associated isolates in Thai clinically healthy pig S. suis strains, although the method works well for human S. suis strains. This assay should be applied to pig S. suis strains with caution. It is highly important that multiplex PCR be validated using more diverse S. suis strains from different geographic areas and origins of isolation.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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