Point-of-care detection of Neisseria gonorrhoeae based on RPA-CRISPR/Cas12a

AMB Express

Original Article

Xiaoying Cao¹  Lili Xiang²  Chao Ling³  Qianrong Tu³  Shifeng Huang³  Ping Yang³ 

[1] Department of Burn and Plastic Surgery, The First Affiliated Hospital of Chongqing Medical University, No. 1 Friendship Road, Yuzhong District, 400016, Chongqing, People’s Republic of China;Department of Clinical Laboratory Medicine, Chongqing Shapingba District Chenjiaqiao Hospital, 401331, Chongqing, People’s Republic of China;Department of Clinical Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, No. 1 Friendship Road, Yuzhong District, 400016, Chongqing, People’s Republic of China;

关键词: Neisseria gonorrhoeae;    Recombinase polymerase amplification;    CRISPR/Cas12a;    Fluorescence;    Lateral flow detection;   

DOI  :  10.1186/s13568-023-01554-7

 received in 2023-04-25, accepted in 2023-05-01,  发布年份 2023

来源: Springer

【 摘 要 】

Gonorrhea, caused by Neisseria gonorrhoeae (N. gonorrhoeae), is a persistent global public health threat. The development of low-cost, point-of-care testing is crucial for gonorrhea control, especially in regions with limited medical facilities. In this study, we integrated CRISPR/Cas12a reaction with recombinase polymerase amplification (RPA) to provide a simple and adaptable molecular detection method for N. gonorrhoeae. The RPA-Cas12a-based detection system developed in this study enables rapid detection of N. gonorrhoeae within 1 h without the use of specialized equipment. This method is highly specific for identifying N. gonorrhoeae without cross-reactivity with other prevalent pathogens. Furthermore, in the evaluation of 24 clinical samples, the detection system demonstrates a 100% concordance rate with traditional culture, which is being used clinically as a reference method. Overall, the RPA-Cas12a-based N. gonorrhoeae detection has the advantages of rapidity, portability, low-cost, no special equipment required, and strong operability, and has a high potential for application as a self-testing and point-of-care diagnosis, which is critical for the clinical management of gonorrhea in developing countries lacking medical equipment.Graphical Abstract

【 授权许可】

CC BY   
© The Author(s) 2023

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