A novel micro-ECoG recording method for recording multisensory neural activity from the parietal to temporal cortices in mice

Molecular Brain

Methodology

Osamu Fukayama¹  Ryota Kanda²  Shuto Tada²  Hiroto Sekiguchi³  Hideyuki Matsumoto⁴  Kenji Mizuseki⁴  Noriaki Ohkawa⁵  Yoshito Saitoh⁵  Mikiko Ishikawa⁵  Takuya Hikima⁵  Susumu Setogawa⁶  Satoshi Nakada⁷  Makoto Osanai⁸  Fumiko Seki⁹  Keigo Hikishima^1,10 

[1] Center for Information and Neural Networks (CiNet), Advanced ICT Research Institute, National Institute of Information and Communications Technology, 565-0871, Suita, Osaka, Japan;Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, 441-8580, Toyohashi, Aichi, Japan;Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, 441-8580, Toyohashi, Aichi, Japan;Japan Science and Technology, Precursory Research for Embryonic Science and Technology (PRESTO), 332-0012, Kawaguchi, Saitama, Japan;Department of Physiology, Osaka Metropolitan University Graduate School of Medicine, 545-8585, Osaka, Japan;Division for Memory and Cognitive Function, Research Center for Advanced Medical Science, Comprehensive Research Facilities for Advanced Medical Science, Dokkyo Medical University, 321-0293, Tochigi, Japan;Division for Memory and Cognitive Function, Research Center for Advanced Medical Science, Comprehensive Research Facilities for Advanced Medical Science, Dokkyo Medical University, 321-0293, Tochigi, Japan;Department of Physiology, Osaka Metropolitan University Graduate School of Medicine, 545-8585, Osaka, Japan;Japanese Center for Research on Women in Sport, Graduate School of Health and Sports Science, Juntendo University, 270-1695, Chiba, Japan;Laboratory for Physiological Functional Imaging, Department of Medical Physics and Engineering, Division of Health Sciences, Osaka University Graduate School of Medicine, 565-0871, Suita, Osaka, Japan;Live Animal Imaging Center, Central Institutes for Experimental Animals (CIEA), 210-0821, Kanagawa, Japan;Medical Devices Research Group, Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 305-8564, Tsukuba, Japan;

关键词: Electrocorticography;    Flexible micro-electrocorticography device;    Multi-sensory;    Cerebral cortex;    Temporal cortex;    Local field potential;   

DOI  :  10.1186/s13041-023-01019-9

 received in 2022-10-11, accepted in 2023-03-09,  发布年份 2023

来源: Springer

【 摘 要 】

Characterization of inter-regional interactions in brain is essential for understanding the mechanism relevant to normal brain function and neurological disease. The recently developed flexible micro (μ)-electrocorticography (μECoG) device is one prominent method used to examine large-scale cortical activity across multiple regions. The sheet-shaped μECoG electrodes arrays can be placed on a relatively wide area of cortical surface beneath the skull by inserting the device into the space between skull and brain. Although rats and mice are useful tools for neuroscience, current μECoG recording methods in these animals are limited to the parietal region of cerebral cortex. Recording cortical activity from the temporal region of cortex in mice has proven difficult because of surgical barriers created by the skull and surrounding temporalis muscle anatomy. Here, we developed a sheet-shaped 64-channel μECoG device that allows access to the mouse temporal cortex, and we determined the factor determining the appropriate bending stiffness for the μECoG electrode array. We also established a surgical technique to implant the electrode arrays into the epidural space over a wide area of cerebral cortex covering from the barrel field to olfactory (piriform) cortex, which is the deepest region of the cerebral cortex. Using histology and computed tomography (CT) images, we confirmed that the tip of the μECoG device reached to the most ventral part of cerebral cortex without causing noticeable damage to the brain surface. Moreover, the device simultaneously recorded somatosensory and odor stimulus-evoked neural activity from dorsal and ventral parts of cerebral cortex in awake and anesthetized mice. These data indicate that our μECoG device and surgical techniques enable the recording of large-scale cortical activity from the parietal to temporal cortex in mice, including somatosensory and olfactory cortices. This system will provide more opportunities for the investigation of physiological functions from wider areas of the mouse cerebral cortex than those currently available with existing ECoG techniques.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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