Titanium biogenic nanoparticles to help the growth of Trichoderma harzianum to be used in biological control

Journal of Nanobiotechnology

Research

Bruno T. Sousa¹  Halley C. de Oliveira¹  Estefânia V. R. Campos²  Leonardo F. Fraceto²  Tatiane Pasquoto-Stigliani³  Renata Lima³  Tais Germano-Costa³  Natalia Bilesky-José³  Bianca B. Migliorini³  Leandro O. Feitosa³  Mariana Guilger-Casagrande⁴ 

[1] Departament of Animal and Plant Biology, University of Londrina (UEL), Londrina, Paraná, Brazil;Institute of Science and Technology of Sorocaba, Laboratory of Environmental Nanotechnology, State University of São Paulo (UNESP), Sorocaba, São Paulo, Brazil;Laboratory for Evaluation of the Bioactivity and Toxicology of Nanomaterials, University of Sorocaba (UNISO), Sorocaba, São Paulo, Brazil;Laboratory for Evaluation of the Bioactivity and Toxicology of Nanomaterials, University of Sorocaba (UNISO), Sorocaba, São Paulo, Brazil;Institute of Science and Technology of Sorocaba, Laboratory of Environmental Nanotechnology, State University of São Paulo (UNESP), Sorocaba, São Paulo, Brazil;

关键词: Nanoparticle biosynthesis;    Green nanoparticle route;    Biological control;    Metal oxide nanoparticles;    Sclerotinia sclerotiorum;    Phytopathogen;   

DOI  :  10.1186/s12951-023-01918-y

 received in 2022-11-04, accepted in 2023-05-04,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe biogenic synthesis of metallic nanoparticles is a green alternative that reduces the toxicity of this nanomaterials and may enable a synergy between the metallic core and the biomolecules employed in the process enhancing biological activity. The aim of this study was to synthesize biogenic titanium nanoparticles using the filtrate of the fungus Trichoderma harzianum as a stabilizing agent, to obtain a potential biological activity against phytopathogens and mainly stimulate the growth of T. harzianum, enhancing its efficacy for biological control.ResultsThe synthesis was successful and reproductive structures remained in the suspension, showing faster and larger mycelial growth compared to commercial T. harzianum and filtrate. The nanoparticles with residual T. harzianum growth showed inhibitory potential against Sclerotinia sclerotiorum mycelial growth and the formation of new resistant structures. A great chitinolytic activity of the nanoparticles was observed in comparison with T. harzianum. In regard to toxicity evaluation, an absence of cytotoxicity and a protective effect of the nanoparticles was observed through MTT and Trypan blue assay. No genotoxicity was observed on V79-4 and 3T3 cell lines while HaCat showed higher sensitivity. Microorganisms of agricultural importance were not affected by the exposure to the nanoparticles, however a decrease in the number of nitrogen cycling bacteria was observed. In regard to phytotoxicity, the nanoparticles did not cause morphological and biochemical changes on soybean plants.ConclusionThe production of biogenic nanoparticles was an essential factor in stimulating or maintaining structures that are important for biological control, showing that this may be an essential strategy to stimulate the growth of biocontrol organisms to promote more sustainable agriculture.Graphical Abstract

【 授权许可】

CC BY   
© The Author(s) 2023

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