Optimization design of interdigitated microelectrodes with an insulation layer on the connection tracks to enhance efficiency of assessment of the cell viability

BMC Biomedical Engineering

Research

Sameh. Sherif¹  Yehya H. Ghallab¹  Yehea Ismail²  Rania Siam³  Omnia AbdelRaheem³  Laila Ziko⁴ 

[1] Biomedical Engineering Department, Helwan University, Cairo, Egypt;Center of Nanoelectronics and Devices (CND), Zewail City of Science and Technology and The American University in Cairo (AUC), Cairo, Egypt;Center of Nanoelectronics and Devices (CND), Zewail City of Science and Technology and The American University in Cairo (AUC), Cairo, Egypt;Department of Biology, School of Sciences and Engineering, The American University in Cairo(AUC), Cairo, Egypt;Department of Biology, School of Sciences and Engineering, The American University in Cairo(AUC), Cairo, Egypt;School of Life and Medical Sciences, the University of Hertfordshire, Hosted By Global Academic Foundation, Cairo, Egypt;

关键词: Impedance;    Microelectrical Impedance Spectroscopy (µEIS);    Microfluidic;    Interdigitated Microelectrodes;    MCF7;    Microbeads;   

DOI  :  10.1186/s42490-023-00070-w

 received in 2022-08-09, accepted in 2023-03-16,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundMicroelectrical Impedance Spectroscopy (µEIS) is a tiny device that utilizes fluid as a working medium in combination with biological cells to extract various electrical parameters. Dielectric parameters of biological cells are essential parameters that can be extracted using µEIS. µEIS has many advantages, such as portability, disposable sensors, and high-precision results.ResultsThe paper compares different configurations of interdigitated microelectrodes with and without a passivation layer on the cell contact tracks. The influence of the number of electrodes on the enhancement of the extracted impedance for different types of cells was provided and discussed. Different types of cells are experimentally tested, such as viable and non-viable MCF7, along with different buffer solutions. This study confirms the importance of µEIS for in vivo and in vitro applications. An essential application of µEIS is to differentiate between the cells’ sizes based on the measured capacitance, which is indirectly related to the cells’ size. The extracted statistical values reveal the capability and sensitivity of the system to distinguish between two clusters of cells based on viability and size.ConclusionA completely portable and easy-to-use system, including different sensor configurations, was designed, fabricated, and experimentally tested. The system was used to extract the dielectric parameters of the Microbeads and MCF7 cells immersed in different buffer solutions. The high sensitivity of the readout circuit, which enables it to extract the difference between the viable and non-viable cells, was provided and discussed. The proposed system can extract and differentiate between different types of cells based on cells’ sizes; two other polystyrene microbeads with different sizes are tested. Contamination that may happen was avoided using a Microfluidic chamber. The study shows a good match between the experiment and simulation results. The study also shows the optimum number of interdigitated electrodes that can be used to extract the variation in the dielectric parameters of the cells without leakage current or parasitic capacitance.

【 授权许可】

CC BY   
© The Author(s) 2023

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