Physiological and transcriptional mechanisms associated with cadmium stress tolerance in Hibiscus syriacus L.

BMC Plant Biology

Research

Komivi Dossa¹  Yanmei Li²  Lu Jia²  Shili Ye³  Lanlan Liu⁴  Yanxuan Yu⁴  Xiang Li⁵  Yunpeng Luan⁶  Shixian Sun⁷ 

[1] CIRAD, UMR AGAP Institut, 34398, Montpellier, France;Department of Life Technology Teaching and Research, School of Life Science, Southwest Forestry University, 650224, Kunming, China;Faculty of Mathematics and Physics, Southwest Forestry University, 650224, Kunming, China;Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, 650224, Kunming, China;The First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine, 650021, Kunming, China;The First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine, 650021, Kunming, China;Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, 650224, Kunming, China;Yunnan Key Laboratory of Plateau Wetland Conservation, Restoration and Ecological Services, Southwest Forestry University, 650224, Kunming, China;

关键词: Hibiscus syriacus;    Cadmium;    Transcriptome;    Antioxidant system;    Differentially expressed genes;    Transcription factors;   

DOI  :  10.1186/s12870-023-04268-x

 received in 2022-10-29, accepted in 2023-05-06,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundCadmium (Cd) pollution of soils is a global concern because its accumulation in plants generates severe growth retardation and health problems. Hibiscus syriacus is an ornamental plant that can tolerate various abiotic stresses, including Cd stress. Therefore, it is proposed as a plant material in Cd-polluted areas. However, the molecular mechanisms of H. syriacus tolerance to Cd are not yet understood.ResultsThis study investigated the physiological and transcriptional response of “Hongxing”, a Cd2+-tolerant H. syriacus variety, grown on a substrate containing higher concentration of Cd (400 mg/kg). The Cd treatment induced only 28% of plant mortality, but a significant decrease in the chlorophyll content was observed. Malondialdehyde content and activity of the antioxidant enzymes catalase, peroxidase, and superoxide dismutase were significantly increased under Cd stress. Transcriptome analysis identified 29,921 differentially expressed genes (DEGs), including 16,729 down-regulated and 13,192 up-regulated genes, under Cd stress. Functional enrichment analyses assigned the DEGs mainly to plant hormone signal transduction, transport, nucleosome and DNA processes, mitogen-activated protein kinase signaling pathway, antioxidant process, fatty acid metabolism, and biosynthesis of secondary metabolites. Many MYB, EP2/ERF, NAC, WRKY family genes, and genes containing metal binding domains were up-regulated, implying that they are essential for the Cd-stress response in H. syriacus. The most induced genes were filtered out, providing valuable resources for future studies.ConclusionsOur findings provide insights into the molecular responses to Cd stress in H. syriacus. Moreover, this study offers comprehensive and important resources for future studies toward improving the plant Cd tolerance and its valorization in phytoremediation.

【 授权许可】

CC BY   
© The Author(s) 2023

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