Speckle-tracking echocardiography provides sensitive measurements of subtle early alterations associated with cardiac dysfunction in T2DM rats

BMC Cardiovascular Disorders

Research

Lixiang Xue¹  Meifang Ma²  Yanchao Qi³  Gang Liu⁴  Yajuan Yin⁵  Yongjun Li⁶  Suyun Liu⁶  Bingyan Guo⁶  Lijie Wang⁶  Zhiyan Chen⁷  Zhe Liu⁷ 

[1] Center of Basic Medical Research, Peking University Third Hospital, 100191, Beijing, People’s Republic of China;Department of Cardiology, Handan Central Hospital, 056008, Handan, Hebei, People’s Republic of China;Department of Cardiology, The First Hospital of Hebei Medical University, 050031, Shijiazhuang, Hebei, People’s Republic of China;Heart Center, The First Hospital of Hebei Medical University, 050031, Shijiazhuang, Hebei, People’s Republic of China;Department of Cardiology, The First Hospital of Hebei Medical University, 050031, Shijiazhuang, Hebei, People’s Republic of China;Heart Center, The First Hospital of Hebei Medical University, 050031, Shijiazhuang, Hebei, People’s Republic of China;Hebei International Joint Research Center for Structural Heart Disease, 050031, Shijiazhuang, Hebei, People’s Republic of China;Hebei Key Laboratory of Cardiac Injury Repair Mechanism Study, 050031, Shijiazhuang, Hebei, People’s Republic of China;Hebei Key Laboratory of Heart and Metabolism, 050031, Shijiazhuang, People’s Republic of China;Department of Cardiology, The First Hospital of Hebei Medical University, 050031, Shijiazhuang, Hebei, People’s Republic of China;Hebei International Joint Research Center for Structural Heart Disease, 050031, Shijiazhuang, Hebei, People’s Republic of China;Hebei Key Laboratory of Cardiac Injury Repair Mechanism Study, 050031, Shijiazhuang, Hebei, People’s Republic of China;Hebei Key Laboratory of Heart and Metabolism, 050031, Shijiazhuang, People’s Republic of China;Department of Cardiology, The Second Hospital of Hebei Medical University, 050000, Shijiazhuang, Hebei, People’s Republic of China;Heart Center, The First Hospital of Hebei Medical University, 050031, Shijiazhuang, Hebei, People’s Republic of China;

关键词: Diabetic cardiomyopathy;    Myocardial damage;    RhoA;    ROCK signalling pathway inhibition;    Cardiac function;    Speckle-tracking echocardiography;   

DOI  :  10.1186/s12872-023-03239-2

 received in 2023-01-09, accepted in 2023-04-12,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundDiabetic cardiomyopathy results in cardiac structural and functional abnormalities. Previous studies have demonstrated that inhibiting the RhoA/ROCK signalling pathway increases the injury resistance of cardiomyocytes. The early detection of cardiac structural and functional alterations may facilitate an improved understanding of the pathophysiologic progress and guide therapy. This study aimed to identify the optimal diagnostic measures for the subtle early alterations of cardiac dysfunction in type 2 diabetes mellitus (T2DM) rats.MethodsTwenty-four rat models were divided into four groups and received treatments for 4 weeks: the CON group (control rats), the DM group (T2DM rats), the DMF group (T2DM rats receiving fasudil) and the CONF group (control rats receiving fasudil) group. Left ventricular (LV) structure was quantified by histological staining and transmission electron microscopy. LV function and myocardial deformation were assessed by high-frequency echocardiography.ResultsTreatment with fasudil, a ROCK inhibitor, significantly protected against diabetes-induced myocardial hypertrophy, fibrosis and mitochondrial dysfunction. Impaired LV performance was found in T2DM rats, as evidenced by significant reductions in the ejection fraction (EF), fractional shortening (FS) and the mitral valve (MV) E/A ratio (which decreased 26%, 34% and 20%, respectively). Fasudil failed to improve the conventional ultrasonic parameters in T2DM rats, but the myocardial deformation measured by speckle-tracking echocardiography (STE) were significantly improved (global circumferential strain, GCS: P = 0.003; GCS rate, GCSR: P = 0.021). When receiver operating characteristic (ROC) curves were used in combination with linear regression analysis, STE parameters were found to be characterized by both optimal prediction of cardiac damage [AUC (95% CI): fractional area change, FAC: 0.927 (0.744, 0.993); GCS: 0.819 (0.610, 0.945); GCSR: 0.899 (0.707, 0.984)] and stronger correlations with cardiac fibrosis (FAC: r = -0.825; GCS: r = 0.772; GCSR: r = 0.829) than conventional parameters.ConclusionThe results suggest that STE parameters are more sensitive and specific than conventional parameters in predicting the subtle cardiac functional changes that occur in the early stage, providing new insight into the management of diabetic cardiomyopathy.

【 授权许可】

CC BY   
© The Author(s) 2023

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