In-vitro pharmacological evaluation of Sulforaphane from Brassica oleracea

TMR Integrative Medicine

article

Sodum Nalini¹  Shaik Chand Basha²  TS Mohamed Saleem³ 

[1] Annamacharya College of Pharmacy;Department of Pharmaceutical Chemistry, Annamacharya College of Pharmacy;Department of Pharmacology, Annamacharya College of Pharmacy

关键词: Sulforaphane;    Anti-arthritic activity;    Protein denaturation methods;   

DOI  :  10.12032/TMRIM201903012

学科分类：医学（综合）

来源: TMR publishing group

【 摘 要 】

Objectives: Sulforaphane has numerous Pharmacological and therapeutic effects like anti-oxidant,anti-cancer, anti-diabetic, anti-arthritic, anti-ulcer, anti-viral. The present study was designed toevaluate the anti-arthritic activity of Sulforaphane by in-vitro screening methods. Methods: Theanti-arthritic activity of Sulforaphane was investigated by protein denaturation assay by usingBovine serum albumin (BSA) and egg albumin. Sulforaphane used in various concentration (10, 50,100,250, 500 ug/ml) against both the methods and Diclofenac sodium was used as referencestandard. Results:The anti-arthiritic activity sulforaphane was directly proportional to inhibition ofdenaturation of albumin. Sulforaphane shows concentration dependent inhibition activity in bothbovine serum albumin and egg albumin assay.The maximum inhibition was observed in theconcentration of 500 ug/ml with 92.8% inhibition for BSA and 96.3% for egg albumin respectively.Conclusion: Sulforaphane have significant anti-arthritic activity accessed by BSA denaturation andegg albumin denaturation assay.Based on present finding future direction also planned toconform the activity by using wellestablished in-vivo methods.

【 授权许可】

CC BY