Treatment of stable vitiligo using cultured and non-cultured autologous melanocytes from hair follicle outer root sheath cell suspension

Journal of the Egyptian Women s Dermatologic Society

article

Heba A. Gad¹  Marwa S. El Hawary²  Hamdy H. Swelim³  Yomna I. Mahmoud³  Shereen M. Shawky⁴ 

[1] Molecular Diagnostic Unit, New Kasr El-Ainy Teaching Hospital;Department of Dermatology, Faculty of Medicine, Cairo University;Department of Zoology, Faculty of Science, Ain Shams University;Department of Clinical Pathology, Faculty of Medicine, Cairo University

关键词: cell suspension;    hair follicle outer root sheath;    melanin content;    melanocytes;    Pmel-17;    transplantation;    vitiligo;   

DOI  :  10.4103/JEWD.JEWD_12_20

学科分类：皮肤病学

来源: Egyptian Women s Dermatological Society

【 摘 要 】

Background  Several surgical treatment methods have been proposed for the treatment of stable vitiligo, but they remain inadequate in terms of patient satisfaction. Objective  To assess the efficacy and safety of a modified autologous cultured hair follicle outer root sheath (HF-ORS) cell suspension transplantation in the treatment of stable vitiligo lesions, and to compare it with transplantation of autologous non-cultured HF-ORS cell suspension for the same patient. Patients and methods  Twenty-four patients were enrolled in this prospective controlled study. Hairs were epilated, and HF-ORS cell suspensions were prepared using two methods for the same patient. The recipient site was prepared by CO2 laser resurfacing. Assessment of cellular activity and viability included cellular melanin content determined by enzyme-linked immunosorbent assay and measurement of the fold change in premelanosome (Pmel-17) gene expression. The patients were followed-up for 6 months postprocedure. Results  Nineteen patients completed the study. The results demonstrated that the cultured method showed superior repigmentation response in 15 (79%) patients, three (16%) responded equally, and one (5%) was more tolerant of the noncultured method. Conclusion  Cultured autologous HF-ORS melanocytes show superior repigmentation response, increased melanin content, and upregulated gene expression of Pmel-17 than that of noncultured ones. However, the study also demonstrated that both methods are potential options for the treatment of stable vitiligo.

【 授权许可】

CC BY