A commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI/SNF complex component

Wellcome Open Research

article

Erin E. Cutts¹  Gillian C. Taylor²  Mercedes Pardo¹  Lu Yu¹  Jimi C. Wills³  Jyoti S. Choudhary¹  Alessandro Vannini¹  Andrew J. Wood² 

[1] Cancer Biology Division, Institute of Cancer Research;MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh;Cancer Research UK Edinburgh Centre, Institute of Genetics and Molecular Medicine, University of Edinburgh

关键词: condensin;    SMC complex;    SWI/SNF;    BAF;    PBAF;    antibody;    specificity.;   

DOI  :  10.12688/wellcomeopenres.16482.1

学科分类：内科医学

来源: Wellcome

【 摘 要 】

Condensin complexes compact and disentangle chromosomes in preparation for cell division. Commercially available antibodies raised against condensin subunits have been widely used to characterise their cellular interactome. Here we have assessed the specificity of a polyclonal antibody (Bethyl A302-276A) that is commonly used as a probe for NCAPH2, the kleisin subunit of condensin II, in mammalian cells. We find that, in addition to its intended target, this antibody cross-reacts with one or more components of the SWI/SNF family of chromatin remodelling complexes in an NCAPH2-independent manner. This cross-reactivity, with an abundant chromatin-associated factor, is likely to affect the interpretation of protein and chromatin immunoprecipitation experiments that make use of this antibody probe.

【 授权许可】

CC BY