Wellcome Open Research | |
Genome-wide CRISPR screening identifies new regulators of glycoprotein secretion | |
article | |
Stephanie Popa1  Julien Villeneuve1  Sarah Stewart1  Esther Perez Garcia2  Anna Petrunkina Harrison2  Kevin Moreau1  | |
[1] University of Cambridge Metabolic Research Laboratories, Wellcome Trust-Medical Research Council Institute of Metabolic Science, University of Cambridge;NIHR Cambridge BRC Cell Phenotyping Hub, Department of Medicine, University of Cambridge | |
关键词: secretion; CRISPR screen; Golgi; endoplasmic reticulum; | |
DOI : 10.12688/wellcomeopenres.15232.2 | |
学科分类:内科医学 | |
来源: Wellcome | |
【 摘 要 】
Background: The fundamental process of protein secretion from eukaryotic cells has been well described for many years, yet gaps in our understanding of how this process is regulated remain.Methods: With the aim of identifying novel genes involved in the secretion of glycoproteins, we used a screening pipeline consisting of a pooled genome-wide CRISPR screen, followed by secondary siRNA screening of the hits to identify and validate several novel regulators of protein secretion.Results: We present approximately 50 novel genes not previously associated with protein secretion, many of which also had an effect on the structure of the Golgi apparatus. We further studied a small selection of hits to investigate their subcellular localisation. One of these, GPR161, is a novel Golgi-resident protein that we propose maintains Golgi structure via an interaction with golgin A5.Conclusions: This study has identified new factors for protein secretion involved in Golgi homeostasis.
【 授权许可】
CC BY
【 预 览 】
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RO202307130000577ZK.pdf | 7700KB | download |