Genome-wide CRISPR screening identifies new regulators of glycoprotein secretion

Wellcome Open Research

article

Stephanie Popa¹  Julien Villeneuve¹  Sarah Stewart¹  Esther Perez Garcia²  Anna Petrunkina Harrison²  Kevin Moreau¹ 

[1] University of Cambridge Metabolic Research Laboratories, Wellcome Trust-Medical Research Council Institute of Metabolic Science, University of Cambridge;NIHR Cambridge BRC Cell Phenotyping Hub, Department of Medicine, University of Cambridge

关键词: secretion;    CRISPR screen;    Golgi;    endoplasmic reticulum;   

DOI  :  10.12688/wellcomeopenres.15232.2

学科分类：内科医学

来源: Wellcome

【 摘 要 】

Background: The fundamental process of protein secretion from eukaryotic cells has been well described for many years, yet gaps in our understanding of how this process is regulated remain.Methods: With the aim of identifying novel genes involved in the secretion of glycoproteins, we used a screening pipeline consisting of a pooled genome-wide CRISPR screen, followed by secondary siRNA screening of the hits to identify and validate several novel regulators of protein secretion.Results: We present approximately 50 novel genes not previously associated with protein secretion, many of which also had an effect on the structure of the Golgi apparatus. We further studied a small selection of hits to investigate their subcellular localisation. One of these, GPR161, is a novel Golgi-resident protein that we propose maintains Golgi structure via an interaction with golgin A5.Conclusions: This study has identified new factors for protein secretion involved in Golgi homeostasis.

【 授权许可】

CC BY