Detection of diverse Wolbachia 16S rRNA sequences at low titers from malaria vectors in Kayin state, Myanmar

Wellcome Open Research

article

Sunisa Sawasdichai¹  Victor Chaumeau¹  Tee Dah¹  Thithiworada Kulabkeeree¹  Ladda Kajeechiwa¹  Monthicha Phanaphadungtham¹  Muesuwa Trakoolchengkaew¹  Praphan Kittiphanakun¹  Yanada Akararungrot¹  Kyi Oo¹  Gilles Delmas¹  Nicholas J. White²  François H. Nosten¹ 

[1]Shoklo Malaria Research Unit, Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University

[2]Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford

[3]Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University

关键词: Wolbachia;    Anopheles;    Plasmodium;    16S rRNA;    entomological inoculation rate;    Southeast Asia;    Kayin state;    wAnga;   

DOI  :  10.12688/wellcomeopenres.15005.4

学科分类：内科医学

来源: Wellcome

【 摘 要 】

Background: NaturalWolbachia infections in malaria mosquitoes were recently reported in Africa, and negatively correlated with the development ofPlasmodium falciparum in the vectors. The occurrence and effects ofWolbachia infections outside Africa have not been described and may have been underestimated.Methods: Mosquitoes were collected by human-landing catch during May and June 2017 in ten villages in Kayin state, Myanmar. Closely related species of malaria vectors were identified with molecular assays. 16S rRNAWolbachia DNA sequences were detected with quantitative real-time PCR.Results: Low titer ofWolbachia DNA was detected in 13/370 samples in six malaria vector species. Sequences were diverse and different from those described in the African malaria mosquitoes.Conclusion: The detection ofWolbachia DNA in malaria mosquitoes from Kayin state warrants further investigations to understand better the ecology and biology ofAnopheles-Wolbachia interactions in Southeast Asia.

【 授权许可】

CC BY