Water source most suitable for rearing a sensitive malaria vector, Anopheles funestus in the laboratory

Wellcome Open Research

article

Genevieve Tchigossou¹  Romaric Akoton¹  Akadiri Yessoufou²  Innocent Djegbe³  Francis Zeukeng¹  Seun M Atoyebi¹  Eric Tossou¹  Kabirou Moutairou²  Rousseau Djouaka¹ 

[1] International Institute of Tropical Agriculture;Laboratory of Cell Biology and Physiology, University of Abomey-Calavi;University of Sciences;Faculty of Science, Department of Biochemistry, University of Yaounde I;Cell Biology and Genetics Unit, Department of Zoology, University of Ibadan

关键词: Anopheles funestus;    rearing;    eggs;    larvae;    F1 generation;    borehole water;    mineral water;    physico-chemical parameters;   

DOI  :  10.12688/wellcomeopenres.12942.2

学科分类：内科医学

来源: Wellcome

【 摘 要 】

Background:  The insecticide susceptibility status ofAnopheles funestus, one of the main malaria vectors in the Afrotropical regions, remains under-studied due to the difficulty of working with this mosquito species. Collecting their larvae in natural breeding sites, rearing and maintaining them in normal laboratory conditions have been a difficult task. Forced-egg laying technique has been a very good tool to generate eggs from adult mosquitoes collected from the wild but rearing these eggs to obtain satisfying portion as adults has always been the problem. In this study, we optimized the development of mosquito species larvae under standard laboratory conditions for desired production of adult mosquitoes that can be useful for insecticide susceptibility tests.Methods:  A forced-egg laying technique was used to obtain eggs from gravid femaleAnopheles funestus collected from Kpome locality in Benin. Eggs were reared in three different water samples (water from the borehole, and two mineral water namely FIFA and Possotômè) and larvae were fed with TetraMin baby fish food. The physico-chemical parameters of the waters were investigated prior to use for egg incubation (introduction of eggs’ batches into water).Results: In contrast to mineral water that had no contamination, the borehole water source was contaminated with lead (2.5mg/L) and nitrate (118.8mg/L). Egg hatching rates ranged as 91.9 ± 4.4%, 89.1 ± 2.5% and 87.9 ± 2.6% in FIFA, Possotômè and borehole water respectively. High emergence of larvae to adult mosquitoes was recorded as in FIFA (74.3%) and Possotômè (79.5%) water. No adult mosquito was obtained from larvae reared in borehole water.Conclusions: This study gave insight on the water sources that could be good for rearing to mass produceAn. funestus in the laboratory. More analysis with other local mineral water sources in our environments could be considered in the future, hopefully giving better outputs.

【 授权许可】

CC BY