Fine-scale spatial and temporal heterogeneities in insecticide resistance profiles of the malaria vector, Anopheles arabiensis in rural south-eastern Tanzania

Wellcome Open Research

article

Nancy S. Matowo¹  Givemore Munhenga¹  Marcel Tanner⁴  Maureen Coetzee¹  Wim F. Feringa⁶  Halfan S. Ngowo²  Lizette L. Koekemoer¹  Fredros O. Okumu² 

[1] Wits Research Institute for Malaria, MRC Collaborating Centre for Multi-disciplinary Research on Malaria, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand;Environmental Health and Ecological Sciences Department, Ifakara Health Institute;Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases;University of Basel;Swiss Tropical and Public Health Institute;Faculty of Geo-Information Science and Earth Observation, University of Twente;Institute of Biodiversity, Animal Health and Comparative Medicine, University of Glasgow;School of Public Health, Faculty of Health Sciences, University of the Witwatersrand

关键词: Spatial and temporal variations;    insecticide resistance;    metabolic resistance;    kdr detection;    malaria control;    Anopheles arabiensis;    Tanzania;   

DOI  :  10.12688/wellcomeopenres.12617.1

学科分类：内科医学

来源: Wellcome

【 摘 要 】

Background: Programmatic monitoring of insecticide resistance in disease vectors is mostly done on a large scale, often focusing on differences between districts, regions or countries. However, local heterogeneities in residual malaria transmission imply the need for finer-scale data. This study reports small-scale variations of insecticide susceptibility inAnopheles arabiensis between three neighbouring villages across two seasons in Tanzania, where insecticidal bed nets are extensively used, but malaria transmission persists.Methods: WHO insecticide susceptibility assays were conducted on female and maleAn. arabiensis from three proximal villages, Minepa, Lupiro, and Mavimba, during dry (June-December 2015) and wet (January-May 2016) seasons. Adults emerging from wild-collected larvae were exposed to 0.05% lambda-cyhalothrin, 0.05% deltamethrin, 0.75% permethrin, 4% DDT, 4% dieldrin, 0.1% bendiocarb, 0.1% propoxur, 0.25% pirimiphos-methyl and 5% malathion. A hydrolysis probe assay was used to screen for L1014F (kdr-w) and L1014S (kdr-e) mutations in specimens resistant to DDT or pyrethroids. Synergist assays using piperonly butoxide (PBO) and triphenol phosphate (TPP) were done to assess pyrethroid and bendiocarb resistance phenotypes.Results: There were clear seasonal and spatial fluctuations in phenotypic resistance status inAn. arabiensis to pyrethroids, DDT and bendiocarb. Pre-exposure to PBO and TPP, resulted in lower knockdown rates and higher mortalities against pyrethroids and bendiocarb, compared to tests without the synergists. Neither L1014F nor L1014S mutations were detected.Conclusions: This study confirmed the presence of pyrethroid resistance inAn. arabiensis and showed small-scale differences in resistance levels between the villages, and between seasons. Substantial, though incomplete, reversal of pyrethroid and bendiocarb resistance following pre-exposure to PBO and TPP, and absence ofkdr alleles suggest involvement of P450 monooxygenases and esterases in the resistant phenotypes. We recommend, for effective resistance management, further bioassays to quantify the strength of resistance, and both biochemical and molecular analysis to elucidate specific enzymes responsible in resistance.

【 授权许可】

CC BY