First detection of N1575Y mutation in pyrethroid resistant Anopheles gambiae in Southern Côte d’Ivoire

Wellcome Open Research

article

Ako Victorien Constant Edi¹  Bedjou Prisca N'Dri¹  Mouhamadou Chouaibou¹  Fondjo Behi Kouadio¹  Patricia Pignatelli⁴  Giovanna Raso²  David Weetman⁴  Bassirou Bonfoh¹ 

[1] Research and Development Department, Centre Suisse de Recherches Scientifiques en Côte d’Ivoire;Swiss Tropical and Public Health Institute;University of Basel;Vector Biology Department, Liverpool School of Tropical Medicine

关键词: Insecticide resistance;    deltamethrin;    N1575 Y mutation;    Anopheles coluzzii;    Anopheles gambiae;    Côte d’Ivoire;    Tiassalé;    Elibou;   

DOI  :  10.12688/wellcomeopenres.12246.1

学科分类：内科医学

来源: Wellcome

【 摘 要 】

Background. The intensification of insecticide use for both public health and agriculture in Africa has contributed to growing insecticide resistance. Today, resistance to World Health Organization (WHO)-approved insecticide classes is widespread. In an agricultural area of Southern Côte d’Ivoire, the main malaria vectorAnopheles coluzzii shows multiple resistance across insecticides mediated by both target site mutation and metabolic mechanisms. To plan new vector control strategies and avert future resistance liabilities caused by cross-resistance mechanisms extant within populations, it is crucial to monitor the development and spread of both resistance and mechanisms.Methods.  Larvae ofAnopheles gambiae were collected from natural breeding sites in Tiassalé and Elibou, between April and November 2016 and raised to adults. Adult female non-blood fed mosquitoes, three to five days old, were exposed to deltamethrin in WHO bioassays. Extracted DNA samples from exposed mosquitoes were used for species characterisation and genotyping.Results. Most adultAn. gambiae tested were resistant to deltamethrin, with mortality rates of only 25% in Tiassalé and 4.4% in Elibou. Molecular analysis of DNA from samples tested showed the presence of bothAn. coluzzii andAn. gambiae s.s in Elibou and onlyAn. coluzzii for Tiassalé. As previously, the L1014Fkdr mutation was present at high frequency (79%) in Tiassalé and the L1014S mutation was absent. The N1575Y mutation, which amplifies resistance conferred by L1014F was detected in a single unique individual from a TiassaléAn. coluzzii female whereas in Elibou 1575Y was present in 10An. gambiae s.s, but not inAn. coluzzii.Conclusion. This is the first report of the N1575Y mutation in Côte d’Ivoire, and as in other populations, it is found in both dominant West African malaria vector species. Continued monitoring of N1575Y is underway, as are studies to elucidate its contribution to the resistance of local vector populations.

【 授权许可】

CC BY