Anti-leishmania Effect of Magnesium Oxide Nanoparticles on Leishmania tropica/infantum and Leishmania -Infected Macrophages

International Journal of Enteric Pathogens

article

Seyyed Hojjat Sadeghi¹  Amir Karimipour-Saryazdi²  Mohammad Mahdi Jafari³  Roya Omidi²  Fatemeh Ghaffarifar² 

[1] Organic and Nano Group ,(ONG), Department of Chemistry, Iran University of Science and Technology;Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University;Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University

关键词: Magnesium oxide;    Leishmania tropica;    Leishmania infantum;    Leishmania-infectedmacrophages;   

DOI  :  10.34172/ijep.2022.5546

学科分类：农业科学（综合）

来源: Alborz University of Medical Sciences

【 摘 要 】

Background: Leishmania is an intracellular protozoan parasite that enters and reproduces inmacrophage cells. Macrophages are important immune cells that phagocyte many pathogenssuch as bacteria, fungi, and parasites such as Leishmania spp. but are incapable of killing thisparasite, living in the phagosomes of infected macrophages, multiplying, and resulting in thedivesting of infected macrophages and the appearance of Leishmania lesions. Many of thepresent drugs for Leishmania treatment have side effects, or parasites have resistance to some ofthese drugs. Therefore, there is a need for a better drug for Leishmania treatment. Magnesiumoxide (MgO) is a metal nanoparticle (NP) with numerous biological applications, includingantioxidant and antimicrobial effects on various pathogens such as some bacteria, fungi, andparasites, including Leishmania spp.Objectives: Accordingly, this article has discussed the effects of MgO NPs on Leishmania tropicaand Leishmania infantum and Leishmania-infected macrophages.Materials and Methods: The effect of various doses of MgO NPs on L. tropica and L. infantumpromastigotes and amastigotes was studied in vitro. Flow cytometry and MTT were also utilizedto assess the cytotoxic effects of MgO on L. tropica and L. infantum promastigotes, as well as thelikelihood of apoptosis. Amastigote assay was employed to determine the infected macrophagepercentage, and the number of parasites present in every macrophage cell.Results: The percentage of macrophages contaminated with amastigotes of L. tropica and L.infantum that were treated with MgO NPs was 15% and 11%, respectively. Flow cytometryrevealed that MgO NPs induced approximately 38.56% and 30.5% apoptosis on L. tropica andLeishmania infantum, respectively. The half maximal inhibitory concentration of MgO NPs to L.tropica and L. infantum according to promastigote assay for 72 hours was 7.32 μg/mL and 12.58μg/mL, respectively.Conclusion: According to the findings, MgO NPs had a great in-vitro fatality effect on L. tropicaand L. infantum promastigotes and amastigotes (inside leishmania-infected macrophages).

【 授权许可】

CC BY-NC