Investigating the Effect of Silver, Chitosan, and Curcumin Nanoparticles on Blastocystis spp. and Comparing it With Metronidazole In Vitro

International Journal of Enteric Pathogens

article

Raheleh Taji¹  Javid Sadraei¹  Majid Pirestani¹  Saeed Bahadory¹ 

[1] Department of Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University

关键词: Blastocystis;    Nanoparticles;    Silver;    Chitosan;    Curcumin;   

DOI  :  10.34172/ijep.2022.5561

学科分类：农业科学（综合）

来源: Alborz University of Medical Sciences

【 摘 要 】

Background: Blastocystis is an anaerobic gastrointestinal protozoan that causes infections inhumans and a wide range of animals. It was found that the host specificity and the pathogenicpotential of different isolates are correlated with sequence variations in the SSU-rRNA gene.The identification of the organism at the species level is still an unclear challenge. The use ofnatural nature substances against infectious organisms has been promising, and the optimizationof these substances in the direction of better delivery such as the form of nanoparticles (NPs) ofnatural substances has recently been considered.Objectives: The present study aimed to investigate the effect of silver, chitosan, and curcuminNPs on Blastocystis spp. and compare it with metronidazole in vitro conditions.Materials and Methods: The parasite was cultivated in Robinson’s medium and was thenidentified by polymerase chain reaction (PCR), and the subtype of the parasite was determined,which was subtype 3. Then, the methyl thiazolyl tetrazolium (MTT) test was performed todetermine the toxicity level of the prepared drugs/substances using Caco2 cells. This studyinvestigated the concentrations of silver NPs (10, 25, and 50 µg/mL), chitosan (75, 50, 25, and12.5 µg/mL), and curcumin (250, 500, and 1000 µg/mL), and their effect on 24- and 48-hourtime points after exposure to the parasite. Then, the final number of parasites was counted afterstaining with trypan blue by a Neubauer slide, and the values of IC50 and selectivity index (SI)were calculated for each substance.Results: Chitosan and curcumin NPs had SI of 2.04 and 13.15, respectively, which were moreeffective than metronidazole, and silver NP was 0.143. However, chitosan NP had the best antiparasitic effect. Based on the obtained results, chitosan and curcumin NPs were more effectiveagainst blastocystis than against metronidazole.Conclusion: Chitosan and curcumin NPs (liposomal curcumin) have a good inhibitory effect onblastocystis compared to metronidazole, but silver NP did not perform better than metronidazole.

【 授权许可】

CC BY-NC