The Effect of Different Concentrations of Methylprednisolone on Survival, Proliferation, and Migration of Neural Stem/Progenitor Cells

Basic and Clinical Neuroscience

article

Zohreh Bagheri¹  Fatemeh Shamsi²  Zahra Zeraatpisheh²  Mahin Salmannejad³  Ahmad Soltani⁴  Hadi Aligholi² 

[1] Department of Physiology, School of Medicine, Shiraz University of Medical Sciences;Department of Neurosciences, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences;Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences;Department of Neurosurgery, Namazi Hospital, Shiraz University of Medical Sciences

关键词: Methylprednisolone;    Neural stem cells;    Proliferation;    Survival;    Migration;   

DOI  :  10.32598/bcn.2021.35.4

来源: Iran University of Medical Sciences

【 摘 要 】

Introduction: The present study addressed whether methylprednisolone (MP) as an anti-inflammatory drug used in neurodegenerative diseases and neural stem/progenitor cells (NS/PCs) is safe.  Methods: First, embryonic rat NS/PCs were exposed to different concentrations of MP, and then we evaluated their survival by MTT assay, proliferation by analyzing the number and diameter of neurospheres, and the migration of the cells by neurosphere assay. Results: The viability of NS/PCs was reduced following exposure to 10, 15, and 20 µg/mL of MP. In addition, although the number of neurospheres did not change, exposure to different concentrations of MP resulted in the formation of smaller neurospheres. Despite these undesirable effects, the highest concentration of MP (20 μg/mL) increased the migration capacity of the NS/PCs. Conclusion: The combination of MP and NS/PCs is not recommended due to the adverse effects of MP on the survival and proliferation of NS/PCs.

【 授权许可】

Unknown